Naslov
Bioprospecting for Genes Encoding Hydrocarbon-Degrading Enzymes from Metagenomic Samples Isolated from Northern Adriatic Sea Sediments

Autori
Gacesa, Ranko ; Baranasic, Damir ; Starcevic, Antonio ; Diminic, Janko ; Korlević, Marino ; Najdek-Dragić, Mirjana ; Blažina, Maria ; Oršolić, Davor ; Kolesarić, Domagoj ; Long, Paul F. ; Cullum, John ; Hranueli, Daslav ; Orlić, Sandi ; Zucko, Jurica ;

Izvornik
Food technology and biotechnology (1330-9862) 56 (2018), 2; 270-277

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, kratko priopcenje, znanstveni

Ključne riječi
oil pollution ; n-alkane degradation, database

Sažetak
Three metagenomic libraries were constructed using surface sediment samples from the northern Adriatic Sea. Two of the samples were taken from a highly polluted and an unpolluted site respectively. The third sample from a polluted site had been enriched using crude oil. The results of the metagenome analyses were incorporated in the REDPET relational database (http://redpet.bioinfo.pbf.hr/REDPET), which was generated using the previously developed MEGGASENSE platform. The database includes taxonomic data to allow the assessment of the biodiversity of metagenomic libraries and a general functional analysis of genes using hidden Markov model (HMM) profiles based on the KEGG database. A set of 22 specialised HMM-profiles was developed to detect putative genes for hydrocarbon-degrading enzymes. Use of these profiles showed that the metagenomic library generated after selection on crude oil had enriched genes for aerobic n-alkane degradation. The use of this system for bioprospecting was exemplified using potential alkB and almA genes from this library.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA