Naslov
ANTIOXIDATIVE AND ANTIDIABETIC EFFECTS OF NARINGIN AND CURCUMIN IN VITRO AND IN VIVO

Autori
Sirovina, Damir ; Odeh, Dyana ; Oršolić, Nada ; Kukolj, Marina ; Nikolić, Barbara ; Lesar, Nikola

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2017 Annual Meeting of the Croatian Immunological Society with EFIS on Tour / - , 2017, 62-62

Skup
2017 Annual Meeting of the Croatian Immunological Society with EFIS on Tour

Mjesto i datum
Zagreb, Hrvatska, 20.10.2017. - 21.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
antioxidative, antidiabetic, naringin, curcuminin

Sažetak
Introduction. Oxidative stress plays an important role in the etiology of diabetes mellitus and can produce damage on cellular macromolecules, such as DNA and lipids in cellular membrane, impair protein function, and trigger cell death. Many polyphenols, exert positive effects on diseases caused by oxidative stress. Curcumin and naringin differ in number and arrangement of the hydroxyl groups, as well as by the nature and extent of alkylation. They can act as reducing agents, free radical scavengers, metal chelators, and singlet oxygen quenchers. The antioxidant activity of curcumin and naringin in vitro are compared with some of their antidiabetic effect in vivo on molecular cellular and organism level. Aim. The aim of the present study was to assess whether naringin or curcumincan influence oxidative stress induced DNA damage in mice with alloxan-induced diabetes. Materials and methods. Diabetes was induced in Swiss albino mice with a single intravenous injection of alloxan at dose of 75 mg kg-1 body weight. Two days after alloxan injection, naringin or curcumin preparations (50 mg kg-1) were given intraperitoneally for 7 days. In order to evaluate the effectiveness of naringin and curcumin we used three assays which cover different aspects of antioxidant activity, observed changes in body weight and survival of mice and used the alkaline comet and micronucleus assays. Results. In vitro curcumin showed appreciable antioxidant properties, while naringin was much less effective. Naringin or curcumin administration to diabetic mice resulted in decreased DNA damage in lymphocytes and increased level of DNA damage in liver, kidney and reticulocytes. Administration of naringin and curcumin resulted in significant increase of the body weight and 100% survival of mice. Conclusion. Results suggests that antioxidant activity of naringin and curcumin leads to long time survival of diabetic mice and possible prevention of further oxidative damage, so they could be candidates for antidiabetic agent, but the precise targets of naringin and curcumin in diabetic mice are still to be clarified.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA