Naslov
Unraveling the hazards of selenium trace elements to earthworm immunity, from genes to cells

Autori
Engelmann, Péter ; Bodó, Kornélia ; Tolnai, Gréta ; Vuković, Rosemary ; Štolfa Čamagajevac, Ivna ; Ečimović, Sandra ; Velki, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, ostalo, znanstveni

Skup
46th Annual Meeting of the Hungarian Society for Immunology

Mjesto i datum
Velence, Mađarska, 18.10.2017. - 20.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
selenium ; earthworm immunity ; gene expression ; coelomocytes

Sažetak
Objectives: It is widely known that selenium (Se) supplementation has beneficial effects on the immune response ; however it also possesses potential risk of toxicity. Its toxicity depends on its chemical form, and applied concentrations. The aim of this study was to estimate the impact of increased concentrations of different forms of Se on the cellular immune components (so called coelomocytes) of earthworms. Methods: Eisenia andrei earthworms were exposed to various concentrations of two different forms of Se, selenite and selenate. By means of flow cytometry Annexin V positivity, caspase 3 activity, and mitochondrial membrane integrity were assessed to evaluate the coelomocyte survival in earthworms exposed to high selenium concentrations. Modulation of immune (TLR, coelomic cytolytic factor, lysenin, lysozyme) and stress-related genes (catalase, Cu/Zn SOD, MnSOD, metallothionein) upon selenium exposure was measured by qPCR. Results: Higher concentrations of selenate caused increased Annexin positivity, induced caspase 3 activity and decreased mitochondrial membrane potential of coelomocytes. Thus, these results indicate an apoptotic-like cell death in the exposed earthworms. In contrast, higher concentrations of selenite evoked a drop off of several immune (lysenin, lysozyme) and stress- related (MT, Cu/ZnSOD, MnSOD) genes in this soil-sentinel organism. Conclusions: Both Se forms showed significant impact on the measured cellular and genetic parameters in earthworms, indicating the disruption of immune and stress homeostasis. Obtained results can serve as basis for further studies on Se effects and will help in including different aspects necessary for understanding of Se impact on cellular immune components of living organisms.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA