Naslov
Decidual macrophages express markers of alternative activation

Autori
Cupurdija, Kristijan ; Hammer, Astrid ; Dohr, Gottfried ; Rukavina, Daniel ; Sedlmayr, Peter

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book / Hrvatsko imunološko društvo - Zagreb : Hrvatsko imunološko društvo, 2002, 34-34

Skup
Annual meeting 2002, Croatian Immunological Society

Mjesto i datum
Trakošćan, Hrvatska, 22.11.2002. - 24.11.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Decidua; Macrophages; Alternative activation; MS-1; Fibronectin; Factor XIIIa

Sažetak
PROBLEM: The decidua, the maternal part of the placenta, consists of different cell types of maternal and fetal origin. The maternal cells consist, among others, of decidual NK cells and macrophages which are abundantly present during early pregnancy ; the fetal part of the decidua consists of invading extravillous cytotrophoblast cells. Interactions of these cells are probably important for the development of fetomaternal tolerance. A few years ago, alternative activation of macrophages has been described.While classically activated macrophages induced by IFN and LPS secrete proinflammatory cytokines, supporting Th1 immune response, alternatively activated macrophages induced by IL-4 and glucocorticoids, IL-10 or IL-13 display an immunosuppressive effect downregulating Th1 response. They have increased capacity for phagocytosis and stimulation of Th2 response and are involved in angiogenesis and downregulation of inflammation. Alternatively activated macrophages are characterized by different marker molecules such as MS-1, coagulation factor XIIIa, fibronectin, IL-10, AMAC-1, prostaglandin E2, IL-1R-antagonist. Our interest is to investigate the hypothesis that classical activation of decidua macrophages leads to pregnancy termination and that alternative activation supports pregnancy. The aim of this study was to establish whether decidual macrophages express markers of alternative activation. METHODS: First trimester decidua tissues were obtained by elective pregnancy terminations. Tissue samples were either snap frozen, cryocut sectioned and acetone-fixed for immunohistology studies, or enzimaticly digested to obtain single cell suspensions for flow cytometry analyses. Cell suspensions were immediately labeled after isolation for detection of MS-1, fibronectin and factor XIIIa. RESULTS: By immunohistology we confirmed the presence of MS-1 in decidual tissue. Double immunofluorescence labeling of the tissue sections analyzed by laser scanning microscopy showed colocalization of MS-1 and CD14. The same results were obtained by flow cytometry of single cell suspensions of decidual cells. Colocalization of fibronectin and CD 14 and factor XIIIa and CD14 was confirmed by flow cytometric analyses of freshly isolated decidual macrophages. CONCLUSION: As decidual macrophages match the phenotype of alternative activation, our data suggest that these cells be alternatively activated and thus may suppress Th1 – type reactions, which in turn may favor pregnancy over abortion. K.C. is supported by Grant from Ministry of Science and Technology, Republic of Croatia (No 062029) and by a grant from the Alps-Adria Association. The project was supported in part by grants from the provincial government of Styria and from the Franz-Lanyar-Stiftung to P.S.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA