Pregled bibliografske jedinice broj: 92008
Appropriate triggering of mannose receptor affects maturation of monocyte derived dendritic cells
Appropriate triggering of mannose receptor affects maturation of monocyte derived dendritic cells // Abstract book / Hrvatsko imunološko društvo (ur.).
Zagreb: Hrvatsko imunološko društvo, 2002. (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 92008 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Appropriate triggering of mannose receptor affects maturation of monocyte derived dendritic cells
Autori
Laškarin, Gordana ; Chieppa, Marcello ; Bianchi, Giancarlo ; Doni, Andrea ; Pasqualini, Fabio ; Marchesi, Federica ; Rukavina, Daniel ; Mantovani, Alberto ; Allavena, Paola
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Abstract book
/ Hrvatsko imunološko društvo - Zagreb : Hrvatsko imunološko društvo, 2002
Skup
Abstract book
Mjesto i datum
Trakošćan, Hrvatska, 22.11.2002. - 24.11.2002
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
-
Sažetak
Introduction.The mannose receptor (MR) is an endocytic receptor belonging to type I C type lectin receptor superfamily. It is expressed on macrophages, myeloid dendritic cells (DC), endothelial cells and Kaposi sarcoma cells. Mannose receptor recognizes surface polysaccharides or glycosilated proteins of several pathogens such as Gram negative, Gram-positive bacteria, yeasts, parasites and mycobacterium and endocyte them. A few number of studies indicates that legation of MR beside endocytosis can induce signals for cell maturation or activation. Due to these properties MR is considered as a pattern recognition receptor involved in host defence and innate immunity. The aim of our study was to examine the effects of specific anti-MR monoclonal antibody clone PAM-1 (PAM-1 MoAb) on the phenotype and function of DC in vitro. Material and methods. Immature DC were differentiated in vitro from monocytes of healthy donors in the presence of GM-CSF (50 ng/ml) and IL-13 (20 ng/ml) during 6 days. To examine phenotype, endocytosis, antigen presenting capacity, IL-10, IL-12 and IL-1 receptor type II (IL-1R type II) secretion of immature DC, immunofluorescency, uptake of Dextran FITC, mixed lymphocyte reaction and ELISA were used, respectively. Further, DC were stimulated with LPS (10 ng/ml), PAM-1 MoAb (IgG1, 2g/ml, “Mario Negri “Institute), anti-MR monoclonal antibody clone 19.2 (19.2 MoAb) (IgG1, 2 g/ml, Pharmingen) and combination of LPS with particular anti-MR antibody in mentioned concentrations. The phenotype and functional characteristic of stimulated DC were analysed and compared with nonstimulated DC. Results. Dendritic cells differentiated in vitro were immature and expressed MR. Staining profile of PAM-1 MoAb and isotype matched 19.2 MoAb was identical, while functional activity of these two antibodies was markedly different. Contrary to 19.2 MoAb, PAM-1 MoAb increases CD83 and CD86 molecule expression and antigen presenting capacity of DC, but decreased their endocytic activity. Dendritic cells treated with PAM-1 MoAb produced more IL-10 and IL-1R type II comparing to untreated DC. PAM-1 MoAb increased IL-10 and decreased IL-12 production in LPS treated DC, while 19.2 MoAb was inefficient. Conclusion. Appropriate triggering of MR by PAM-1 MoAb induces maturation program in DC characterized by distinct pattern of cytokine production.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
