Naslov
Progesteron decreases perforin release from decidual NK cells in a close contact with K562 targets

Autori
Dorčić, Dorotea ; Laškarin, Gordana ; Štrbo, Nataša ; Sotošek Tokmadžić, Vlatka ; Bogović-Crnčić, Tatjana ; Ćupurdija, Kristijan ; Juretić, Koraljka ; Randić, Ljiljana ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Croatian Immunological Society Annual meeting : Abstract book / - Zagreb : Hrvatsko imunološko društvo, 2002, 36-363

Skup
Croatian Immunological Society Annual meeting

Mjesto i datum
Trakošćan, Hrvatska, 22.11.2002. - 24.11.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
decidual NK cells; progesterone; PIBF; perforin degranulation

Sažetak
During normal pregnancy tissue of fetal origin-trophoblast develops and differentiats within uterus in the close contact with maternal tissue. Maternal decidua is infiltrated with immunocompetent leukocytes of specific phenotype and function. Decidual NK cells are predominant cell population with a phenotype CD56++CD16-. These cells show characteristics of activated cells and express cytolytic protein-perforin in their cytoplasmic granules. Release of perforin from granules is carefuly controled. Progesterone Induced Blocking Factor (PIBF), produced by progesterone receptor positive cells, mediates progesterone effects and support Th2 type shift in the cytokine production. Dominance of Th2 cytokines in normal early pregnancy deciduas down-regulate cell-mediated immunity. The aim of our study was to analyze possible effects of progesterone and PIBF on cell mediated cytotoxicity by analysing perforin release from decidual NK cells in vitro. Studies were performed on the suspension of freshly isolated decidual cells that were incubated for 18 hrs with progesterone, PIBF or in the medium only. Cytolytic activity of decidual lymphocytes (DL) as non-adherent cell fraction was measured by PKH-26 (red) 2 hrs cytotoxicity assay against NK sensitive K-562 cell line. DL pretretment with Concanamycin A was used to evaluate the contribution of perforin-mediated cytotoxicity. Perforin protein was analysed by flow cytometry in the suspension of DL treated with progesterone, PIBF or cultured in the medium alone, at the beginning and at the and of 2 hr cytotoxicity assay in the effector/target ratio 50:1. Perforin degranulation from DL was analysed by immunocytochemistry. Concanamycin A, blocator of perforin egzocytosis decreases cytolytic activity of DL against K-562 cell line. Progesterone, as well as PIBF, on a dose dependent manner decreases cytolytic activity of DL against K-562 cell line. Percentage of perforin positive cells at the end of 2 hr citotoxicity assay in the effector/target ratio 50:1 decreases in the suspension of DL, while remains stabile in the suspension of progesterone- or PIBF- treated DL comparing to the beginning of the assay. The average number of perforin molecule per cell was always significantly higher in the suspension of progesterone- or PIBF-treated DL than in nontreated DL at the end of 2 hr cytotoxicity assay. Progesterone specificaly, by its mediator PIBF decreases cytolytic activity of decidual NK cells against K-562 targets by preventing degranulation and keeping perforin inside the cytoplasmatic granulas.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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