Naslov
Oxidative and antioxidative status of nuchal muscles in rats exposed to chronic and acute stress

Autori
Vuković, Ana ; Vitić, Zorica ; Vuković, Rosemary ; Štolfa Čamagajevac, Ivna ; Blažetić, Senka ; Viljanac, Branka ; Balog, Marta ; Has- Schön, Elizabeta, Heffer, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
RECOOP 12th Bridges in Life Sciences Annual Conference / - Budimpešta, 2017, 64-64

Skup
Bridges in life Sciences, 12th Annual Scientific conference - RECOOP HST ASSOCIATION

Mjesto i datum
Budimpešta, Mađarska, 06.04.2017. - 09.04.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
acute stress, chronic stress, nuchal muscles, oxidative stress, ovariectomy
(acute stress ; chronic stress ; nuchal muscles ; oxidative stress ; ovariectomy)

Sažetak
Acute and chronic stress can induce the production of reactive oxygen species which exert a multitude of biological effects that ranges from physiological regulatory functions to damaging alterations and thus participating in the pathogenesis of a number of diseases. In order to estimate the impact of acute and chronic stress on oxidative and antioxidative response in nuchal muscles (Semispinalis capitis, Splenius capitis, Splenius cervicis) of male (M), female (NON-OV) and ovariectomized female (OV), Sprague-Dawley rats were exposed to acute and chronic stress protocol. As an indicator of oxidative damage, lipid peroxidation levels expressed in terms of thiobarbituric acid reactive substances (TBARS) were determined, while antioxidative status was determined by catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), glutathione S- transferase (GST) activities and oxidized/reduced glutathione (GSH/GSSG) ratio. Both acute and chronic stress significantly increased TBARS content in in nuchal muscles of all groups relative to control groups. Chronic stress decreased antioxidative defence mechanism in nuchal muscles of males and females. Acute stress did not have significant impact on the most of the antioxidative enzyme activities (CAT, GPX, GST, SOD and GR) in males and females, although GSH/GSSG ratio was reduced. Ovariectomy increased LPO and reduced some of antioxidative enzyme activities (CAT, GPX). Oxidative and antioxidative response to acute and chronic stress, in nuchal muscles of males and females, showed similarity in most of the measured parameters, and it differed from the response in ovariectomized females. Unlike acute stress, chronic stress disrupts antioxidative defence significantly more, thus it can cause changes in muscle contraction and muscle fatigue, which may lead to chronic pain in neck and headache.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

POVEZANOST RADA