Naslov
Biomarkers of PTSD: preliminary data on glycomic biomarkers and aging

Autori
Pivac, Nela ; Tudor, Lucija ; Konjevod, Marcela ; Švob Štrac, Dubravka ; Nikolac Perković, Matea ; Nedić Erjavec, Gordana ; Uzun, Suzana ; Kozumplik, Oliver ; Lauc, Gordan

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FENS Regional Meeting : Book of abstracts / - , 2017

Skup
FENS Regional Meeting

Mjesto i datum
Pečuh, Mađarska, 20.09.2017. - 23.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
PTSD ; Glycomics ; Aging ; biomarkers

Sažetak
Post-traumatic stress disorder (PTSD) is trauma- and stressor-related disorder in which direct exposure to a traumatic experience/ stressful event(s) leads to an onset of re- experiencing, avoidance, numbing and hyper- arousal symptoms in some but not all subjects exposed to trauma. Its etiology is far from clear. Alterations in various biological systems (HPA, immune, noradrenergic, serotonergic systems), together with interactions with different psychological, social, environmental, genetic and epigenetic factors, contribute to development of PTSD. At present, there are no specific, sensitive and validated biomarkers for PTSD. Glycosylation of proteins results in a formation of specific glycans that significantly affect protein structure and function, especially immunoglobulin G (IgG) molecules and is essential in inflammatory cascade. N-glycans are altered in pediatric and adulthood diseases of the CNS. PTSD is frequently complicated with different somatic diseases and characterized by the increased pro-inflammatory state, while N- glycosilation changes are associated with inflammatory processes and aging. Accelerated aging and altered 9 N-glycan structures were reported in a study including a small number of trauma exposed subjects. Present study evaluated total plasma and IgG N-glycan changes related to PTSD and/or aging in 204 subjects with current and chronic PTSD and 134 control subjects without traumatic experience. Results (39 chromatographic peaks of plasma N-glycans and 24 chromatographic peaks of IgG Ngycans) are presented as total area normalized values (% of each glycan peak /GP/ area in total chromatogram area) and were evaluated using Mann Whitney test.Results showed 16 increased and 10 decreased plasma N-glycan peaks, and 9 increased and 6 decreased IgG N-glycan peaks in PTSD participants compared to control subjects, confirming significant N-glycome changes in PTSD. As age considerably affects N-glycans, GlycoAge test =log(10) GP1/GP10 revealed significant case-control differences in total (U=8329.00 ; p=1.24-09), as well as in older (60-77 years) PTSD sample (U=341.00 ; P=0.022) in plasma N-glycan peaks. Significantly increased plasma GP2 in total PTSD cases and decreased plasma GP11 in older PTSD subjects was related to aging. Moreover, significantly higher G4 and G6 peaks and significantly lower G14 peak in total PTSD and in the oldest PTSD sample versus controls were found for IgG N-glycan associated with aging. These results confirmed N-glycan changes related to aging in PTSD.PTSD and N-glycans are defined by complex and dynamic interactions between genes and environment. As glycan structures are adaptable and respond to environmental stimuli, changes in specific N- gycans in PTSD subjects, which were correlated with aging, suggest their role in the aging process, might be used as biomarkers, and should be further validated and replicated.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA