Naslov
Encapsulation of new biologically active peptides into liposomes and their immunoadjuvant effect

Autori
Frkanec, Ruža ; Tomašić, Jelka ; Vranešić, Branka ; Halassy Špoljar, Beata ; Krstanović, Marina

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
Peptides 2002, Proceedings of the Twenty-Seventh European Peptide Symposium / Benedetti, Ettore ; Pedone, Carlo - Napulj : Edizioni Ziino, 2002, 490-491

Skup
Twenty-Seventh European Peptides Symposium

Mjesto i datum
Sorrento, Italija, 31.08.2002. - 06.09.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
liposomes; peptidoglycan monomer; adamantyltripeptides; adjuvants

Sažetak
We have been examining the encapsulation of the new immunomodulators of peptidoglycan structure and synthetic adamantyltripeptides into the liposomes with the aim to possibly increase and optimize their therapeutic efficacy. Our studies concern two groups of immunomodulators: a) peptidoglycan monomer (PGM, GlcNAc-MurNAc-L-Ala-D-isoGln-mesoA2pm(eNH2)-D-Ala-D-Ala) and b) synthetic adamantyltripeptides (D- and L-(adamant-2-yl)-Gly-L-Ala-D-isoGln) comprising a part of peptidoglycan structure. All mentioned compounds were shown previously to exhibit immunostimulating activity1, 2. The immunostimulators tested were incorporated in large multilamellar negatively charged vesicles using egg lecithin and synthetic dipalmitoyl phosphatidylcholine (DPPC). The entrapment of tested compounds and the stability of the respective preparations were followed in two ways: by the use of 14C-labelled peptidoglycans or by HPLC. The immunoadjuvant activity was tested in vivo, in mice, using ovalbumin (OVA) as an antigen. The production of OVA-specific antibodies was monitored by ELISA. In comparison to the nonentrapped tested compounds the encapsulated preparations showed the better anti-OVA antibody production. Hence, incorporation of immunoadjuvants into liposomes improved their adjuvant activity. In conclusion, liposomes containing immunoadjuvants were prepared, quantity of incorporated material determined and the improved adjuvant effect of immunoadjuvants was demonstrated. 1. J. Tomašić, I. Hanzl-Dujmović, B. Špoljar, B. Vranešić, M. Šantak, A. Jovičić, Vaccine 8 (2000) 1236-1243. 2. B. Vranešić, J. Tomašić, S. Smerdel, D. Kantoci, F. Benedetti, Helv. Chim. Acta 76 (1993) 1752-1758.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA