Naslov
FCS testing of DNA interaction with cyclodextrine based supramolecular systems functionalized with adamantyl guanidine

Autori
Štimac, Adela ; Tokić, Matea ; Ljubetič, Ajasja ; Vuletić, Tomislav ; Frkanec, Leo ; Šekutor, Marina ; Frkanec, Ruža

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
12th Christmas Biophysics Workshop : Book of abstracts / Rozman, Jan ; Ziherl, Primož - Ljubljana, 2017, 8-8

Skup
12th Christmas Biophysics Workshop

Mjesto i datum
Golte, Slovenija, 11.12.2017. - 12.12.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Recenziran

Ključne riječi
adamantyl guanidine ; amphiphilic beta-cyclodextrine ; supramolecular complexes, liposomes, host-guest interaction ; fluorescence correlation spectroscopy

Sažetak
The aim of the present study was preparation of cyclodextrine based supramolecular systems functionalized with adamantyl guanidine and probing of their interaction with DNA using fluorescence correlation spectroscopy (FCS). We prepared and characterized supramolecular systems based on phosphatidylcholine liposome and vesicles of amphiphilic β-cyclodextrine (CD) derivative functionalized by entrapment of a series of adamantyl guanidines AG 1-5. Efficiency of incorporation of adamantyl guanidines into prepared supramolecular systems was measured spectrophotometrically, and the size and surface charge were determined by dynamic light scattering method. It was shown that all examined compounds were successfully incorporated into prepared supramolecular systems except compound AG5. The highest encapsulation efficiency was demonstrated for compound AG1. The incorporation of adamantyl guanidines did not affect the size of prepared vesicles but the vesicle charge was significantly changed. FCS was applied to examine the interactions between functionalized vesicles and the Cy5- fluorescently labelled, double-stranded, 120 bp DNA (DNA120*). The obtained results have clearly demonstrated that the vesicles with entrapped AG 1-4 present guanidinium groups on the surface of vesicles, which leads to interaction with DNA120* via guanidine- phosphate interaction. Also, it was shown that vesicles modified with adamantyl guanylhydrazones (AG 1-2) bind DNA more efficiently, for almost an order of magnitude than vesicles modified with adamantyl aminoguanidines (AG 3-4). Preliminary results have shown that prepared functional supramolecular systems strongly bind DNA and as such could be used in gene delivery.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA