Pregled bibliografske jedinice broj: 909482
FixK2, a key regulator inBradyrhizobium japonicum, is a substrate for the protease ClpAP in vitro
FixK2, a key regulator inBradyrhizobium japonicum, is a substrate for the protease ClpAP in vitro // FEBS letters, 587 (2012), 1; 88-93 doi:10.1016/j.febslet.2012.11.014 (međunarodna recenzija, članak, znanstveni)
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Naslov
FixK2, a key regulator inBradyrhizobium japonicum,
is a substrate for the protease ClpAP in vitro
Autori
Bonnet, Mariette ; Stegmann, Monika ; Maglica, Željka ; Stiegeler, Emanuel ; Weber-Ban, Eilika ; Hennecke, Hauke ; Mesa, Socorro
Izvornik
FEBS letters (0014-5793) 587
(2012), 1;
88-93
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Clp protease ; CRP/FNR superfamily ; FixK2 regulated proteolysis ; transcription factor
Sažetak
FixK2 is a CRP-like transcription factor that controls the endosymbiotic lifestyle of Bradyrhizobium japonicum. The reason for its noticeable protease sensitivity was explored here. The repertoire of Clp chaperone-proteases in B. japonicum was examined, and specifically ClpAP1 and ClpXP1 were purified and tested. FixK2 was found to be degraded by ClpAP1 but not by ClpXP1. Degradation was inhibited by the ClpS1 adaptor protein, indicating that FixK2 is a direct substrate for ClpAP1. The last 12 amino acids of FixK2 appeared to be recognized by ClpA. The results suggest that the ClpAP system is involved in the cellular turnover of FixK2.
Izvorni jezik
Engleski
Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Poljoprivreda (agronomija)
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
