Naslov
Polymorphism of cwd susceptible coons at red deer population in Croatia.

Autori
Zecevic E, Curik I, Dokso A, Rustempasic A, Cubric-Curik V, Brka M

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Proceedings Of The 24Th International Scientific-Expert-Conference Of Agriculture And Food Industry,

Mjesto i datum
Sarajevo, Bosna i Hercegovina, 25.09.2013. - 28.09.2013

Vrsta sudjelovanja
Ostalo

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
alleles, DNA, genes, genetic polymorphism, genomes, genotypes, haplotypes, polymerase chain reaction, populations, prions, wasting disease

Sažetak
Chronic Wasting Disease (CWD) belongs to the group of transmissible spongiform encephalopathy (TSE), caused by prions. CWD is well known in deer population in North America more than 30 years. This disease shortens life period of animals and it is contagious. In this work 10 codons of exon 3 PrP gene has been investigated. As material genomic DNA has been used, and PCR amplification of specific regions of DNA by specially designed primers (forward 5′- ATTTTGCAGATAAGTCATC-3′ and reverse 5′- AGAAGATAATGAAAACAGGAAG-3′) (Peletto et al. 2009) was done. These are the codons that are found in the literature as suspicious for disease occurrence: 26, 78, 95, 96, 98, 116, 132, 136, 138 and 225. Eight genotypes and eight haplotypes have been found. All genotypes were resistant to CWD. Prevailed wild type of alleles, which is understandable considering that for these animals was not present artificial selection.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Biotehnologija

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