Naslov
Comparison of sampling procedures for the detection of Chlamydiaceae in free-range chicken flocks

Autori
Quilicot, Ana Marquiza M. ; Horvatek Tomić, Danijela ; Gottstein, Željko ; Lukač, Maja ; Prukner-Radovčić, Estella

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 7th International Congress ''Veterinary Science and Profession'' / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2017, 8-8

Skup
7th International Congress ''Veterinary Science and Profession''

Mjesto i datum
Zagreb, Hrvatska, 05.10.2017. - 07.10.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Chlamydiaceae, chicken, free-range, sampling procedures

Sažetak
Different sampling procedures were employed in evaluating poultry flocks for the prevalence of avian chlamydiosis based on the detection of Chlamydiaceae by real-time polymerase chain reaction (qPCR) assay. The presence and detection of Chlamydia in samples can be influenced by the type of avian sampled and the length of time of infection. This study compared three sampling procedures used for the detection of Chlamydiaceae in free-range poultry flocks. Triple swabs (conjunctival, pharyngeal and cloacal), litter- fecal and water samples were collected from 30 free-range chicken flocks in Croatia and in the Philippines. Detection of Chlamydiaceae was done by qPCR assay targeting the Ch23S rRNA gene. The detection rate with litter-fecal samples (25/30 ; 83.33% positive) was found to be significantly higher than triple swabs (11/30 ; 36.67% ; p<0.01) and water samples (18/30 ; 60.00% ; p<0.05). Detection rate in water samples is also significantly higher than triple swab samples (p<0.05). Based on these results, litter-fecal sample combination can be examined for the presence of Chlamydiaceae especially if it is difficult to collect an optimum number of samples from free- range chickens to represent a flock for epidemiological studies. However, it is suggested that multiple sampling procedures be performed in evaluating flocks for the presence of Chlamydiaceae regardless of whether infection is acute or chronic.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA