Naslov
Specific and highly efficient condensation of GC and IC DNA by polyaza pyridinophane derivatives

Autori
Radić Stojković, Marijana ; Gonzalez-Garcia, Jorge ; Šupljika, Filip ; Galiana-Rosello, Cristina ; Guijarro, Lluis ; Gazze, Salvatore A. ; Francis, Lewis W. ; Piantanida, Ivo ; Garcia- Espana, Enrique

Izvornik
International journal of biological macromolecules (0141-8130) 109 (2018); 143-151

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Pyridinophane compounds ; DNA/RNA binding ; GC-DNA condensation ; circular dichroism spectroscopy

Sažetak
Two bis-polyaza pyridinophane derivatives and their monomeric reference compounds revealed strong interactions with ds-DNA and RNA. The bis-derivatives show a specific condensation of GC- and IC-DNA, which is almost two orders of magnitude more efficient than the well-known condensation agent spermine. The type of condensed DNA was identified as psi-DNA, characterized by the exceptionally strong CD signals. At variance to the almost silent AT(U) polynucleotides, these strong CD signals allow the determination of GC-condensates at nanomolar nucleobase concentrations. Detailed thermodynamic characterisation by ITC reveals significant differences between the DNA binding of the bis- derivative compounds (enthalpy driven) and that of spermine and of their monomeric counterparts (entropy driven). Atomic force microscopy confirmed GC-DNA compaction by the bis-derivatives and the formation of toroid- and rod-like structures responsible for the psi-type pattern in the CD spectra.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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