Naslov
Construction of novel Saccharomyces cerevisiae hybrid strains resistant to growth and fermentation inhibitors

Autori
Štafa, Anamarija ; Pranklin, Andrea ; Zandona, Antonio ; Žunar, Bojan ; Svetec Miklenić, Marina ; Šantek, Božidar ; Svetec, Ivan Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Biotechnology, Special Issue / Dundar, Munis - , 2017, 15-15

Skup
European Biotechnology Congress 2017

Mjesto i datum
Dubrovnik, Hrvatska, 25.05.2017. - 27.05.2017

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
yeast hybrids, growth and fermentation inhibitors

Sažetak
Bioethanol production from raw lignocellulosic material requires a producer strain which tolerates both the presence of growth and fermentation inhibitors and high ethanol concentrations. Therefore, we constructed heterozygous (hybrid) diploid S. cerevisiae strains by mating two haploid isolates having those desirable traits. In comparison to parental haploids, hybrid diploids were more resistant to several inhibitors frequently found in different lignocellulosic hydrolysates, such as acetic and levulinic acid and 2-furaldehyde. Interestingly, hybrids showed different fermentation ability in a CO2 production test. Our results suggest that construction of intraspecies hybrids coupled with the use of different genetic engineering techniques is a promising approach for the improvement or development of new biotechnologically relevant strains of S. cerevisiae. Additionally, we also show that in two biotechnologically interesting natural isolates percentage of successful gene targeting (gene targeting fidelity) is much lower than in widely used laboratory strains. Furthermore, the most frequent off-targeted event was duplication of a targeted chromosome yielding aneuploids having two copies of targeted chromosome, one containing transformed and the other containing untransformed allele. These results warn us that it could be more difficult to make precise genetic modifications of natural S. cerevisiae strains than expected.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA