Naslov
Exogenous isolation of antibiotic and metal resistance plasmids from pharmaceutical wastewaters released into the environment

Autori
Gonzalez Plaza, Juan Jose ; Blau, Khald ; Šimatović, Ana ; Milaković, Milena ; Bielen, Ana ; Ahel, Marijan ; Smalla, Kornelia ; Udiković Kolić, Nikolina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
4th THETheodor Escherich Symposium on Medical Microbiome Research : Abstract Book / - , 2017, 6-6

Skup
Theodor Escherich Symposium 2017

Mjesto i datum
Graz, Austrija, 12.10.2017. - 13.10.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Exogenous plasmid transfer ; Horizontal gene transfer ; Antibiotic resistance

Sažetak
Antibiotic resistance (AR) is currently one of the most significant epidemiological risks. It is linked to anthropogenic activities, as release of pharmaceutical industry wastewaters, which often contain high concentrations of antibiotics and metals. Release into aquatic environment is assumed to promote dissemination of antibiotic resistance genes (ARGs) among environmental bacteria, and potentially to human pathogens. We need more information to understand the mobilization of ARGs in the environment influenced by antibiotic pollution to control the emergence of resistant pathogens. Studies of sediments at discharge points of two pharmaceutical factories in Croatia showed a high proportion of antibiotic resistant bacteria (ARB), which may lead to increased AR in human pathogens by horizontal gene transfer (HGT). Conjugation is one of most important HGT mechanisms for the spread of ARGs. Once established on plasmids, resistance genes may spread across different strains, species, or genera. Moreover, multiple ARGs are often co-localized on the same plasmid (coresistance), allowing for the spread of multidrug resistance. We have assayed whether plasmids containing ARGs genes from pharmaceutical discharge sites are transferable to other bacteria through exogenous plasmid transfer. Wastewater or sediments influenced by antibiotic pollution, as well as sediments from upstream and downstream sites, were mixed with model recipient E. coli. Transconjugants were selected based on AR markers and green fluorescent signal emission. Frequency of transconjugation was calculated for each site. Besides, we have characterized the presence/absence of ARGs as well as other metal/biocide resistance genes through qPCR and PCR, and antibiogram profiles for unique isolates were determined.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti

POVEZANOST RADA