Naslov
Pregnancy induced changes of cytotoxic activity of conventional and extrathymic lymphatic cells in mice.

Autori
Šimin, Marija ; Radić, Davor ; Mrakovčić-Šutić, Ines ; Ćuk, Mira ; Radošević-Stašić, Biserka ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
American Journal of Reproductive Immunology / Gleicher, Norbert - Chicago (IL) : The American Society for Reproductive Immunology, 2001, 101-101

Skup
VIII International Congress of Reproductive Immunology

Mjesto i datum
Opatija, Hrvatska, 02.07.2001. - 06.07.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
syngeneic pregnancy; Liver

Sažetak
Problem: The immune compromise in decidua allows a semiallogeneic fetus to survive without impairing the ability of the maternal immune system to fight infections. Success of pregnancy depends on fetal trophoblast cells, which form the interface with the mother and express monomorphic, non classical HLA or MHC class I antigens, like CD1d or HLA-G, which protects cells against natural killer (NK) cell lysis and against allo-cytotoxic T lymphocyte responses. Peripheral maternal immunity, however, remains, generally preserved, although during pregnancy and early puerperium an immune balance in favor of suppression was noted, as well an immunomodulation of cytokine responses towards the Th2 paradigm. A specific function in these events seems to have also extrathymically generated NK and NKT cells, which show synchronous expansion in the liver and in the pregnant uterus. Method of study: To elucidate the pregnancy-induced changes in the mother at the sites of extrathymic and conventional T cell generation, in this study we made phenotypic and functional characterization of maternal mononuclear lymphatic cells in the liver, thymus and spleen, using FACS analysis and testing their cytotoxicity against NK and NKT sensitive targets. Experiments were performed in both syngeneic and allogeneic combinations of mating. Results: The data showed that maternal liver in the late phase of syngeneic pregnancy contains more NK1.1.+ and CD4+ cells than have non-pregnant mice. In the spleen an increased proportion of CD3+ and CD4+ cells was also noticed. Preliminary data also showed that cytotoxicity of splenic cells, obtained from mice at the 16th day of syngeneic pregnancy against NK sensitive Yac-1 targets is markedly increased. Conclusion: The data point to the liver as a site of extrathymic T cell generation in both types of pregnancies, and emphasize that changes in the cytotoxic activities of maternal NK and NKT cells, found in the syngeneic pregnancy, might be linked not only with semiallogeneic nature of the fetus, but also with other physiological functions of innate and adaptive immunity.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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