Pretraga

Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 904094

Effect of α cypermethrin on chromosome mis segregation and aneuploidy induction in human peripheral blood lymphocytes as measured by FISH coupled micronucleus assay

Mužinić, Vedran; Želježić, Davor
Effect of α cypermethrin on chromosome mis segregation and aneuploidy induction in human peripheral blood lymphocytes as measured by FISH coupled micronucleus assay // Molecular Cytogenetics: Meeting abstracts from the 11th European Cytogenetics Conference / Iourov, Ivan (ur.).
Firenca, Italija: BioMed Central, 2017. str. 72-72 doi:10.1186/s13039-017-0319-3 (predavanje, međunarodna recenzija, sažetak, znanstveni)

CROSBI ID: 904094 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Effect of α cypermethrin on chromosome mis segregation and aneuploidy induction in human peripheral blood lymphocytes as measured by FISH coupled micronucleus assay

Autori
Mužinić, Vedran ; Želježić, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Molecular Cytogenetics: Meeting abstracts from the 11th European Cytogenetics Conference / Iourov, Ivan - : BioMed Central, 2017, 72-72

Skup
11th European Cytogenetics Conference

Mjesto i datum
Firenca, Italija, 01.07.2017. - 04.07.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
α-cypermethrin ; fluorescence in situ hybridization ; chromosomes 9, 18, X and Y

Sažetak
Aneuploidy results from chromosome loss, gain or mis-segregation during mitosis, and is the most commonly observed type of genomic instability in cancer. We have tested α-cypermethrin, a pyrethroid insecticide widely used in modern agriculture, for its ability to lead to mis-segregation of chromosomes in anaphase and induce aneuploidy in human peripheral blood lymphocytes. Two concentrations relevant for real scenario exposure, one in terms of residual exposure level (0, 0036 μg/ml) and another a common concentration of 3 μg/ ml, were tested. After treatment according to cytokinesis-block micronucleus assay, nuclei of binucleate cells were analyzed by fluorescence in situ hybridization (FISH) using centromeric, directly labelled probes for chromosomes 9, 18, X and Y looking for presence of the selected chromosomes. The results have shown that both concentrations of α-cypermethrin significantly affected the ratio of missegrated sex chromosomes (up to 94.5% for X and up to 96.5% for Y chromosome compared to 35.5% and 71.5% in the negative controls). No effect on somatic chromosomes was observed. However, we detected a decrease in the occurence of aneuploidy regarding both sex chromosomes (up to 4.5% for X and 3.5% for Y compared to 40% and 20% in the negative controls, respectively), and chromosome 18 at the higher concentration tested (7.5% compared to 14.0%). Overall, we conclude that α-cypermethrin has across the tested concentrations significant effect on chromosome missegregation and aneuploidy induction for chromosomes X, Y and 18.

Izvorni jezik
Engleski



POVEZANOST RADA

Projekti:
HRZZ-IP-2013-11-8366 - Organska zagađivala u okolišu - markeri i biomarkeri toksičnosti (OPENTOX) (Želježić, Davor, HRZZ - 2013-11) ( CroRIS)

Profili:

Avatar Url Vedran Mužinić (autor)

Avatar Url Davor Želježić (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Mužinić, Vedran; Želježić, Davor
Effect of α cypermethrin on chromosome mis segregation and aneuploidy induction in human peripheral blood lymphocytes as measured by FISH coupled micronucleus assay // Molecular Cytogenetics: Meeting abstracts from the 11th European Cytogenetics Conference / Iourov, Ivan (ur.).
Firenca, Italija: BioMed Central, 2017. str. 72-72 doi:10.1186/s13039-017-0319-3 (predavanje, međunarodna recenzija, sažetak, znanstveni)
Mužinić, V. & Želježić, D. (2017) Effect of α cypermethrin on chromosome mis segregation and aneuploidy induction in human peripheral blood lymphocytes as measured by FISH coupled micronucleus assay. U: Iourov, I. (ur.)Molecular Cytogenetics: Meeting abstracts from the 11th European Cytogenetics Conference doi:10.1186/s13039-017-0319-3.
@article{article, author = {Mu\v{z}ini\'{c}, Vedran and \v{Z}elje\v{z}i\'{c}, Davor}, editor = {Iourov, I.}, year = {2017}, pages = {72-72}, DOI = {10.1186/s13039-017-0319-3}, keywords = {α-cypermethrin, fluorescence in situ hybridization, chromosomes 9, 18, X and Y}, doi = {10.1186/s13039-017-0319-3}, title = {Effect of α cypermethrin on chromosome mis segregation and aneuploidy induction in human peripheral blood lymphocytes as measured by FISH coupled micronucleus assay}, keyword = {α-cypermethrin, fluorescence in situ hybridization, chromosomes 9, 18, X and Y}, publisher = {BioMed Central}, publisherplace = {Firenca, Italija} }
@article{article, author = {Mu\v{z}ini\'{c}, Vedran and \v{Z}elje\v{z}i\'{c}, Davor}, editor = {Iourov, I.}, year = {2017}, pages = {72-72}, DOI = {10.1186/s13039-017-0319-3}, keywords = {α-cypermethrin, fluorescence in situ hybridization, chromosomes 9, 18, X and Y}, doi = {10.1186/s13039-017-0319-3}, title = {Effect of α cypermethrin on chromosome mis segregation and aneuploidy induction in human peripheral blood lymphocytes as measured by FISH coupled micronucleus assay}, keyword = {α-cypermethrin, fluorescence in situ hybridization, chromosomes 9, 18, X and Y}, publisher = {BioMed Central}, publisherplace = {Firenca, Italija} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Social Science Citation Index (SSCI)
  • Scopus
  • MEDLINE

Citati:

    Altmetrijski pokazatelji:


    Podijeli:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font
    CROSBI koristi kolačiće (cookies) kako bi poboljšao funkcionalnost stranice.