Naslov
Study of nucleic acids interactions of novel asymmetric dicationic monomethine cyanine dyes as potential markers for mitochondrial DNA

Autori
Orehovec, Iva ; Kurutos, Atanas ; Crnolatac, Ivo ; Tomašić Paić, Ana ; Piantanida, Ivo ; Gadjev, Nikolai ; Deligeorgiev, Todor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
RICT 2016, Interfacing Chemical Biology and Drug Discovery / - , 2016, 168-168

Skup
52. International Conference on Medicinal Chemistry

Mjesto i datum
Caen, Francuska, 06.07.2016. - 08.07.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Cijaninske boje, fluorescentni markeri, mitohondrijska DNA, spektroskopija
(Cyanine dyes, fluorescent probes, mitochondrial DNA, cell biology, spectroscopy)

Sažetak
The cyanine analogues are the most used fluorescent dyes which have made a mayor impact in the field of fluorescent probes due to low toxicity, remarkable fluorescence enhancement upon DNA/RNA binding and specific or selective affinity to certain base pair sequences. Here we present spectroscopy methods we have used for studying interactions of five novel asymmetric dicationic monomethine cyanine dyes with DNA/RNA. Spectroscopy studies revealed that AK-A dyes bind with micromolar affinity to all ds-DNA/RNA, intercalating into ds-DNAs and forming aggregates within ds-RNA major groove. Some dyes also show strong fluorimetric selectivity between various ds-DNA and ds-RNA, dependent on a type of polynucleotide secondary structure. AK-A dyes specifically accumulate in mitochondria and have negligible antiproliferation activity on human carcinogenic lung (H460) and breast cancer (MCF-7) cells.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA