Naslov
Validation of chromatographic method for monitoring of photolytic degradation of hydroxychloroquine

Autori
Babić, Sandra ; Dabić, Dario ; Pek, Elvira

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
13. International conference "Laboratory Competence" / - , 2017, 1-1

Skup
13. International conference "Laboratory Competence"

Mjesto i datum
Poreč, Hrvatska, 04.10.2017. - 07.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Hydroxychloroquine ; Validation ; HPLC-DAD ; Photolytic degradation

Sažetak
Pharmaceuticals are used for the purpose of preventing or treating diseases in humans and animals. The consequence of using pharmaceuticals is their presence in the environment. After release in the environment pharmaceuticals may be exposed to abiotic (hydrolysis and photolysis) and biotic (biodegradation) processes. These processes lead to the reduction of pharmaceuticals concentration in the environment. Furthermore, the mentioned processes could result in formation of products that are more or less stable and toxic than the parent compound. Therefore, it is important to obtain knowledge about pharmaceutical fate and behaviour in the environment. In this work, the HPLC-DAD method for monitoring of photolytic degradation of hydroxychloroquine was validated. Validation was performed using ultra pure and spring water. Method limits of quantification (LOQ) were 0.09 mg/L and 0.19 mg/L for ultra pure and spring water, respectively. The method was shown to be linear (R2>0.995) over the concentration range of (LOQ - 12.5) mg/L. Precision and trueness were investigated at three concentration levels (0, 3 mg/L, 4 mg/L and 11 mg/L) over the linear range in both matrices. The results of the research have shown that the method is acceptable for monitoring of photolytic degradation of hydroxychloroquine in spring water.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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