Naslov
Haptoglobin Glycopeptides Analysis by nano-LC-ESI- MS/MS

Autori
Šimunović, Jelena ; Stavenhagen, Kathrin ; Pezer, Marija ; Lauc, Gordan ; Wuhrer, Manfred

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
EuPA School on Practical Proteomics

Mjesto i datum
Split, Hrvatska, 08.10.2017. - 12.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
haptoglobin ; glycosylation ; glycopeptides ; mass spectrometry

Sažetak
Haptoglobin (Hp) is a positive acute phase protein synthesized by hepatocytes. It is a tetramer, which always contains 2 β and 2 α chains, connected via disulfide bonds. Hp has four N-glycosylation sites at Asn184, Asn207, Asn211 and Asn241, located on the β chain and occupied by complex type N-glycans. Its glycosylation, as one of the most significant posttranslation modifications, is associated with different kinds of diseases. In addition, Hp glycosylation is a promising target in cancer research. Our aim was to analyze Hp N- glycosylation in a site-specific manner on glycopeptide level by nano-LC-ESI-MS/MS. Thus, we optimized a method with Hp standard, which was treated with trypsin and a combination of trypsin and endoproteinase Glu-C, in order to obtain glycopeptides containing a single glycosylation site. Data analysis showed that we obtained four glycopeptides with each one glycosylation site, when combining both enzymes. However, to obtain a quantitative digestion with only singly glycosylated species, sample preparation before nano-LC-ESI- MS/MS analysis will be further optimized.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA