Naslov
Relation of antioxidant status at admission and disease severity and outcome in dogs naturally infected with Babesia canis canis

Autori
Crnogaj, Martina ; Cerón, José Joaquin ; Šmit, Iva ; Kiš, Ivana ; Gotić, Jelena ; Brkljačić, Mirna ; Matijatko, Vesna ; Peres Rubio, Camila ; Kučer, Nada ; Mrljak, Vladimir

Izvornik
BMC Veterinary Research (1746-6148) 13 (2017), 114; 114, 9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Babesiosis, dog, antioxidant status, glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase, total antioxidant status (TAS)

Sažetak
Canine babesiosis is caused by species of the Babesia genus and has become an emerging disease worldwide. To the authors’ knowledge there are no reports in which antioxidants have been analyzed in different presentations of canine babesiosis or in which the prognostic value of antioxidants has been studied. The aim of this study was to evaluate whether oxidative stress could be related to the severity and outcome of canine babesiosis. For this purpose a profile consisting of four antioxidant biomarkers (superoxide dismutase - SOD, glutathione peroxidase - GPx, catalase, total antioxidant status - TAS) and malondialdehyde - MDA as an oxidant biomarker (previously evaluated, here studied for comparative purposes) were evaluated in dogs with canine babesiosis of different clinical severity and outcomes. The study was conducted with a sample of 40 dogs suffering from babesiosis (further divided into uncomplicated, one complication and multiple organ dysfunction syndrome - MODS group) and 30 healthy dogs (control group). Additionally, the babesiosis group was divided according to the anaemia into non-anaemic, mildly anaemic, moderately anaemic and severely anaemic dogs. The results of our study showed significantly decreased SOD, catalase and TAS values in diseased dogs compared to controls, while there were no significant differences in GPx between these groups. Dogs that developed MODS showed lower activities of SOD and GPx and higher MDA values compared to dogs with uncomplicated babesiosis as well as with dogs that developed one complication. Superoxide dismutase, catalase and GPx were negatively correlated whereas MDA was positively correlated with the lethal outcome of the disease. Furthermore, this study detected more pronounced decrease in antioxidant biomarkers (SOD, GPx and catalase) in dogs with moderate anaemia compared to those with mild anaemia. The results of this study showed changes in biomarkers related to the antioxidant status of dogs naturally infected with B. canis canis. These biomarkers could be used as indicators of disease severity and outcome in dogs suffering from babesiosis.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
This research was carried out at laboratories of Faculty of Veterinary Medicine, University of Zagreb and Croatian Veterinary Institute and partly financial supported by Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, whereas party by the FP7 ERA Chair project “VetMedZg” (Grant Agreement No 621394). All data generated or analyzed during this study are included in thisFP7 ERA Chair project “VetMedZg”.

POVEZANOST RADA