Naslov
Detection of histamine in fish by surface enhanced Raman spectroscopy using solid silver SERS substrates

Autori
Janči, Tibor ; Ivanda, Mile ; Mikac, Lara ; Marušić, Nives ; Medić, Helga ; Vidaček, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
5th Trans-Atlantic Fisheries Technology conference (45th WEFTA meeting) "Towards a better use of aquatic resources" / - , 2015, 60-60

Skup
5th Trans-Atlantic Fisheries Technology conference (45th WEFTA meeting) "Towards a better use of aquatic resources"

Mjesto i datum
Nantes, Francuska, 12.10.2015. - 15.10.2015

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
histamine, Surface Enhanced Raman Spectroscopy, quality, fish

Sažetak
Histamine fish poisoning is common problem that occurs worldwide and is probably the most frequent form of intoxication caused by consumption of seafood. Although large number of methods for laboratory histamine testing and laboratory screening of fish and related products has been developed over time they are often time and labor consuming and require expensive equipment and skilled personnel to conduct analysis. Consequently, there is still need for more simplified, fast methods suitable for short time on-field histamine testing of fish and related products. Surface Enhanced Raman spectroscopy offers possibility of short-time analysis of different analytes at low concentration with minimal sample preparation. In our previous study, histamine has been detected using silver colloid SERS substrates in fish muscle extracts at concentration of 1x10-4 M which conforms to 111 mg/kg of fish. However, achieved level of detection is slightly higher than legislative set limits for histamine and results showed significant variations due to large number of factors influencing acquisition of SERS spectra, such as aggregation of silver nanoparticles and adsorption of histamine and other molecules present in extract on the aggregated nanoparticles. In order to minimize variations of results and lower the limit of detection, Ag “film over nanospheres” (FON) solid SERS substrates which are known to have very uniform SERS enhancement of >107, were examined in this study. Ag FON substrates were prepared by drop-coating of polystyrene nanospheres on a clean glass microscope coverslips which after drying formed close-packed array of nanospheres on the surface of coverslip. Ag was thermally evaporated in vacuum and deposited atop nanosphere arrays to form SERS active surface. Several size of polystyrene nanospheres and Ag film thickness were examined in order to achieve optimal histamine SERS signal enhancement. Results obtained with different Ag FON substrates in histamine water solutions and histamine spiked fish muscle extracts will be presented.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA