Naslov
Detection of histamine in fish by Surface Enhanced Raman Spectroscopy using silver colloid SERS substrates

Autori
Janči, Tibor ; Ivanda, Mile ; Mikac, Lara ; Petrak, Tomislav ; Marušic, Nives ; Medić, Helga ; Vidaček, Sanja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
44th WEFTA meeting "Seafood science for a changing demand / - , 2014

Skup
44th WEFTA meeting "Seafood science for a changing demand

Mjesto i datum
Bilbao, Španjolska, 09.06.2014. - 11.06.2014

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
histamine, fish, SERS, Raman spectroscopy

Sažetak
In recent years, there has been increasing interest in the use of spectroscopic methods such as Raman spectroscopy for fast and non- invasive evaluation of food safety and quality. Histamine poisoning occurs throughout the world and is probably the most frequent form of intoxication caused by consumption of fish. Therefore, the potential of Surface Enhanced Raman Spectroscopy (SERS) to detect histamine in water solution and fish muscle extract was investigated in this study. Normal Raman spectra of histamine in solid state and SERS spectra of histamine in solutions were collected using 2 different lasers with excitation wavelengths of 532 and 514, 5 nm. Four different silver colloids were used as SERS substrates. Colloids were prepared by chemical reduction of silver nitrate using trisodium citrate, dextran, sodium borohydride and ascorbic acid as reducing agents. Combination of each of these colloids with different aggregating agents (KNO3, KBr, KCl and NaBH4) added in different concentrations has been investigated in order to obtain maximum SERS signal of histamine. Best results were obtained using citrate reduced silver colloid with addition of 0, 23 M NaBH4 enabling detection of histamine in water solution at concentration of 5*10-5 M. Fish muscle extracts were prepared by homogenization of fish muscle in 0, 4 M perchloric acid and spiked with different concentrations of histamine in order to record histamine spectra. Direct spectra acquisition from extract was not possible probably due to impact of perchloric acid on silver colloid and high background noise generated by other extracted components of fish muscle. After purification with anion exchange resin histamine has been detected in fish muscle extract at concentration 1*10-4 M. Although further improvements are needed to achieve detection of histamine at legislative set limits this preliminary results show a potential for development of new histamine detection method in fish and fish products.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA