Naslov
Intramammary propolis solution (Apimast) for subclinical mastitis treatment in dairy cows

Autori
Šuran, Jelena ; Milošević, Karla ; Galović, Mihael ; Mašek, Tomislav ; Brozić, Diana ; Matanović, Krešimir ; Radin, Lada ; Aladrović, Jasna ; Šeol Martinec, Branka ; Radić, Božo ; Radić, Saša ; Benić, Miroslav ; Kostelić, Antun ; Božić, Frane ; Bačić, Goran ; Maćešić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts 7th International Congress "Veterinary Science and Profession" / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2017, 66-66

ISBN
978-953-8006-13-5

Skup
7th International Congress "Veterinary Science and Profession"

Mjesto i datum
Zagreb, Hrvatska, 05.10.2017. - 07.10.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
subclinical mastitis, dairy cows, propolis, apimast, intramammary

Sažetak
Subclinical mastitis is the most important cause of milk production loss and one of the biggest economic problems in dairy farming. Usually, it is not being treated, mainly due to costs of withdrawal time that requires milk discard. The aim of this research was to investigate the safety and efficacy of intramammary propolis solution (Apimast) on subclinical mastitis in dairy cows, since propolis is effective against many gram-positives and has no withdrawal time. Apimast was prepared as a 1% and 3% propolis in non-alcoholic carrier. Concentrations of 8 biomarkers of propolis were determined by high performance liquid chromatography with diode-array detection (HPLC-DAD). In vitro susceptibility (MIC) of common mastitis pathogens to Apimast was determined using agar dilution method. The safety and efficacy study was conducted on five dairy farms of Holstein's cattle and included 86 dairy cows or 339 quarters. The quarters were subsequently classified into groups depending on the SCC (somatic cell count) values (>200.000/ml or <200.000/ml) and the results of the bacteriological finding prior to the application (infected or non-infeted). Eight propolis biomarkers were determined in Apimast ; caffeic, p-coumaric, ferulic and cinnamic acid, quercetin, kaempferol, apigenin and chrysin. The in vitro antimicrobial effect of Apimast was good ; MIC90 for S. aureus was 64, and for S. uberis was 32 µg/ml. Apimast increased SCC values in all animals after the second administration, but these values returned to normal after two days. Additionally, after third administration of Apimast, bacteriological healing was achieved in total of 92.5 % of cases in only 7 days. The results of this study have shown that Apimast, a solution without withdrawal period and a good tolerance, could be used to treat subclinical mastitis and thus, represents a significant innovation in dairy production medicine.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA