Naslov
Analytical validation of canine kidney injury molecule-1 (KIM-1) immunoassay in urine samples

Autori
Kuleš, Josipa ; Nižić, Petra ; Beer Ljubić, Blanka ; Barić Rafaj, Renata ; Mrljak, Vladimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th International congress "Veterinary science and profession", Book of abstracts / Brkljača Bottegaro, Nika ; Zdolec, Nevio ; Vrbanac, Zoran - Zagreb, 2017, 129-129

ISBN
978-953-8006-13-5

Skup
7th International congress "Veterinary science and profession"

Mjesto i datum
Zagreb, Hrvatska, 05.10.2017. - 07.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
validation ; ELISA ; kidney injury

Sažetak
The kidney injury molecule-1 (KIM-1) is a novel biomarker for prediction, diagnosis and prognosis of acute kidney injury. The aim of this study was to evaluate commercially available canine-specific KIM-1 ELISA (Immunology Consultants Laboratory, Inc., Portland, USA) for use in canine urine samples. For assay precision, two pools of urines with different concentrations of analyte that corresponded to low and high ranges of the assay were prepared from samples obtained from dogs. Intra-assay coefficient of variation (CV) was calculated, after analysis of the high and low pool, six times in a single assay run. Inter- assay CV was determined by analysing the same sample in five separate runs carried out on different days. Two samples with different amount of analyte were mixed in different ratios to perform a spiking recovery test. Test recovery, expressed as a percentage, was calculated for each dilution for comparison of expected versus measured concentrations. To investigate the effects of haemolysis, lipiduria and bilirubinuria, canine pools were mixed with different concentrations of haemoglobin, lipid or bilirubin solution. Intra- and inter-assay coefficients of variation (CV) for assay were both below 10%, i.e. for low range pool CV was 9, 98% (mean ± SD: 12, 03 ± 1, 2 ng/ml) ; for high range pool 8, 83% (62, 57 ± 5, 53 ng/ml) ; and for inter- assay 8, 39% (13, 81 ± 1, 16 ng/ml). The recovery between observed and expected concentrations ranged from 94 – 108%, with a mean of 101%. The different degrees of bilirubinuria tested in this study did not affect the measured concentrations of KIM-1 in the canine urine samples, while significant increase in KIM-1 concentrations was found in the presence of severe haemolysis (> 5 g/l) and also in marked lipiduria (triglycerides > 5 g/l). This method exhibited acceptable analytical characteristics, allowing its use in the laboratory with an adequate precision and recovery, with exception of highly haemolysed and/or lipuric urine samples.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

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