Naslov
Design of Small Molecular Probes for Proteins and DNA / RNA

Autori
Piantanida, Ivo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 10th Joint Meeting on Medicinal Chemistry / Basarić, Nikola ; Namjesnik, Danijel ; Perković, Ivana ; Stepanić, Višnja - Zagreb : Hrvatsko kemijsko društvo, 2017, 41-41

ISBN
978-953-55232-8-4

Skup
10th Joint Meeting on Medicinal Chemistry

Mjesto i datum
Srebreno, Hrvatska, 25.06.2017. - 28.06.2017

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Nije recenziran

Ključne riječi
DNA recognition ; protein recognition ; fluorescence ; CD ; aggregation

Sažetak
Small molecules targeting DNA, RNA and/or proteins have attracted significant scientific interest not only because of their biomedicinal applications, but also due to widespread use of spectrophotometric markers in the related scientific research. For instance, fluorescent techniques significantly developed during the last three decades and now represent about 70% of the detection enabling technologies used in molecular biology and medicine. However, design of small molecule (Mw<600) for recognition of ds-DNA/RNA sequence or particular protein is very challenging due limited number of modifications in such restricted molecule size. Quite often, small modifications lead to change of target preference, for instance from DNA-targeting to protein-targeting molecule One of our research lines is focused on the generally under-investigated approach: exploitation of intrinsic property of some dyes for aggregation, whereby monomeric and aggregated dye differ strongly not only in target recognition but also in spectroscopic properties1. Thus, one dye molecule could bind with similar affinity to several targets (DNA, RNA, protein) giving different spectroscopic responses for each target: to some polynucleotide sequence dye would bind as monomer, for other sequence as dimer, and protein binding site would induce different spectroscopic response (Scheme 1). The ongoing research endeavours to establish for the low molecular weight dyes the structure-activity guidelines for fine tuning of DNA - RNA - protein preferences combined with recognition by set of sensitive and bio-applicable spectrometric methods.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA