Naslov
Isolation and identification of Yersinia enterocolitica strains from food production chain

Autori
Zdolec, Nevijo ; Dobranić, Vesna ; Kazazić, Snježana ; Šimunić, Ivan ; Dumbović, Zvonimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of 7th International Congress "Veterinary Science and Profesion" / Brkljača Bottegaro, Nika ; Zdolec, Nevijo ; Vrbanac, Zoran - Zagreb : Veterinarski fakultet Sveučilišta u Zagrebu, 2017, 99-99

ISBN
978-953-8006-13-5

Skup
7th International Congress "Veterinary Science and Profesion"

Mjesto i datum
Zagreb, Hrvatska, 05.10.2017. - 07.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Yersinia enterocolitica ; food chain ; MALDI-TOF MS ; chromogenic media

Sažetak
Human foodborne yersiniosis is mostly associated with consumption of raw or undercooked food of animal origin. During a three-year period, we tested 91 thermally processed and fermented meat products, 32 fresh meat and meat preparations at the end of their shelf-life, 295 raw milk and 9 unpasteurized milk cheeses sampled at retail level, and 78 pig tonsils sampled during slaughter processing, for the presence of Yersinia enterocolitica. Samples were enriched in PSB and ITC broth, and then streaked on CIN agar and CHROMagarTM Y.enterocolitica. Presumptive colonies were identified by MALDI-TOF MS. Pathogenic Y. enterocolitica was absent in raw milk, unpasteurized milk cheeses, thermally processed and fermented meat products. Y. enterocolitica was recovered from 26 tonsils (33.33 %) and two fresh pork meat samples from retail (6.25 %). A total of 80 presumptive colonies were selected from CIN/CHROMAgar and all were identified as Y. enterocolitica by means of MALDI-TOF MS. The results demonstrate the low occurrence of pathogenic Y. enterocolitica in dairy products and processed meats on the market. On the other hand, the persistence of pathogen in the pig population is a risk factor for meat contamination during slaughter processing and meat cutting. The combination of chromogenic culture media and MALDI -TOF MS may be used as alternative method for faster isolation and confirmation of pathogenic Y. enterocolitica from food chain samples.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

Napomena
Istraživanje je financiralo Sveučilište u Zagrebu, kratkoročna potpora istraživanju "Izolacija i karakterizacija patogenih Yersinia enterocolitica u lancu proizvodnje domaćih i divljih svinja" (voditelj: Nevijo Zdolec).

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