Naslov
Prevalence and antimicrobial resistance of Campylobacter spp found in gulls, Laridae, wintering in Zagreb, Croatia

Autori
Jurinović, Luka ; Humski, Andrea ; Šimpraga, Borka ; Kompes, Gordan ; Mikulić, Marina ; Krstulović, Fani1

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
19th International workshop on Campylobacter, Helicobacter and Related Organisms / - Nantes, 2017, 315-315

Skup
International workshop on Campylobacter, Helicobacter and Related Organisms

Mjesto i datum
Nantes, Francuska, 10.09.2017. - 14.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Campylobacter, galebovi, antimikrobna rezinstencija
(Campylobacter, Gulls, antimicrobial resistance)

Sažetak
A total of 173 gulls of 4 species (Yellow- legged Gull, Larus michahellis ; Black-headed Gull, L. ridibundus ; Caspian Gull, L. cachinanns, and Common Gull, L. canus) were captured using cannon net, in a period between November 2016 and March 2017 on Zagreb city garbage dump (45.45 N 16.01 E) in order to collect cloacal swabs. Taken swab samples were tested for the presence of thermophillic Campylobacter spp. using EN ISO 10272-1 method while the species determination was performed by multiplex PCR. Highest prevalence was found in Common Gulls (33, 33 %) followed by Black- headed Gulls (22, 89 %), Caspian Gulls (20, 00 %) and Yellow-legged Gulls (18, 37 %). Among 39 positive samples the most prevalent was C. jejuni (31 or 79, 5 %) followed by C. lari (7 or 18, 0 %) and C. coli (1 or 2, 5 %). To our knowledge this is the first data on occurrence of Campylobacter lari in Croatia. Antimicrobial susceptibility testing was determined for six antimicrobials (erythromycin, ciprofloxacin, tetracycline, gentamicin, nalidixic acid and streptomycin) by microdilution method (SensititreTM EUCAMP2, Thermo scientific) on 15 C. jejuni, 7 C. lari and 1 C. coli isolated strains. EUCAST ECOFFs values and EURL-AR interpretative criteria were used for C. jejuni and C. coli, while for C. lari same criteria as for C. coli were used. All of the tested isolates were resistant to ciprofloxacin, streptomycin and nalidixic acid. All of the C. jejuni and C. coli isolates were resistant to gentamicin, while 53, 3 % of C. jejuni and 28, 6 % of C. lari were resistent to erytromycin. Furthermore, 22, 2 % of C. jejuni were resistant to tetracycline. The observed multi-resistance of the thermophillic Campylobacter contributes thesis of the importance of gull species as important reservoir of Campylobacter and as a source of possible infection for animals and humans.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA