Naslov
Plasma N-glycome composition can identify individuals at increased risk of type 2 diabetes development

Autori
Keser, Toma ; Gornik, Ivan ; Vučković, Frano ; Lukić, Edita ; Lauc, Gordan ; Gornik, Olga

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
17th FEBS Young Scientists’ Forum (YSF)

Mjesto i datum
Jeruzalem, Izrael, 07.09.2017. - 10.09.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Diabetes predisposition ; Hyperglycaemia ; N-linked glycans ; Plasma N-glycome ; Type 2 diabetes

Sažetak
Better understanding of type 2 diabetes and its prevention is a pressing need. Changes in human plasma N-glycome are associated with many diseases and represent promising diagnostic and prognostic biomarkers. Variations in glucose metabolism directly affect glycosylation through the hexosamine pathway, but studies of plasma glycome in type 2 diabetes are limited. We hypothesize that glycans can predict the development of type 2 diabetes. Using chromatographic approach, we analyzed N-linked glycans from plasma proteins of 59 patients who developed hyperglycemia during ICU hospitalization due to an acute illness (a known predictor of type 2 diabetes) and compared them with glycans from 49 similar ICU patients who remained normoglycemic. Samples were taken after the cessation of inflammatory process was confirmed (based on blood count and CRP). Individuals at higher risk of diabetes presented increased branching of plasma N- glycans. Using total plasma N-glycome data, we created a mathematical algorithm with good diagnostic power (sensitivity and specificity 96.6 % and 85.4 %, respectively) to identify people at increased risk for development of type 2 diabetes. Plasma protein N-glycosylation has a good ability to identify people at increased risk of type 2 diabetes. This might offer a potentially new approach for improvement in diabetes prevention, and consequent reduction in the incidence of this widespread disease.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti, Farmacija

POVEZANOST RADA