Naslov
An outbreak caused by multiresistant Acinetobacter baumannii with rarely found sequence type 502 and OXA-40 carbapenemase

Autori
Goić-Barišić, Ivana ; Šeruga Musić, Martina ; Kovačić, Ana ; Rubić, Žana ; Novak, Anita ; Tonkić, Marija ; Hrenović ; Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
11th International Symposium on the Biology of Acinetobacter / - Sevilla, 2017, 120-120

Skup
11th International Symposium on the Biology of Acinetobacter

Mjesto i datum
Sevilla, Španjolska, 20.09.2017. - 22.09.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
bacteria, public health, infection

Sažetak
Since 2009, University Hospital of Split (UHS) in Croatia has a growing problem in number of infections caused by carbapenem-resistant isolates of A. baumannii, now almost endemically present in most of intensive care units inside the hospital. Here, we report a new outbreak in the Neurology Intensive Care Unit (NICU) that started in March 2017, after a transfer of patient from a hospital in a neighbouring state. First isolate of A. baumannii with unusual resistant pattern (susceptible to imipenem but resistant to meropenem) was collected in the beginning of March 2017, from tracheal aspirate patient with brain stroke transferred from General Hospital of Livno (Bosnia and Herzegovina). This isolate was resistant to meropenem, gentamicin, amikacin, ciprofloxacin, levofloxacin and trimethoprim/sulfamethoxazole, but susceptible (MIC 1.5 mg/L) to imipenem, colistin (0.19 mg /L) and ampicillin/sulbactam (2 mg/L). In the next eight weeks, 10 new isolates with same resistant pattern were isolated, also from respiratory specimens (tracheal aspirates and BALs) from NICU and Pulmonary Department inside University Hospital of Split. Isolates were collected and subjected to further multilocus sequence typing analyses according to MLST (Oxford scheme). The MLST results have revealed that all isolates belong to the ST (sequence type) 502, within the clonal complex 92 and IC 2, very rarely described in the literature, and for the first time detected in Croatia. Multiplex polymerase chain reaction (PCR) using specific primers for blaOXA-51-like, blaOXA-40-like, blaOXA-23-like, blaOXA-58-like and blaOXA-143-like genes was performed and the presence of OXA-51-like and OXA-40-like oxacillinase in selected isolates was confirmed. Further sequencing of amplified fragments will show the similarity of the new sequence type of carbapenem resistant A. baumannii with the endemic IC 2 clone carrying OXA-72 oxacillinase.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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