Naslov
Human Mesenchymal Stem Cells Differentiation Regulated by Hydroxyapatite Content within Chitosan-Based Scaffolds under Perfusion Conditions

Autori
Rogina, Anamarija ; Antunović, Maja ; Pribolšan, Lidija ; Caput Mihalić, Katarina ; Vukasović, Andreja ; Ivković, Alan ; Marijanović, Inga ; Gallego Ferrer, Gloria ; Ivanković, Marica ; Ivanković, Hrvoje

Izvornik
Polymers (2073-4360) 9 (2017), 9; 387, 17

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Chitosan ; Hydroxyapatite ; HMSC’s ; Perfusion-bioreactor
(chitosan ; hydroxyapatite ; hMSC’s ; perfusion-bioreactor)

Sažetak
The extensive need for hard tissue substituent greatly motivates development of suitable allogeneic grafts for therapeutic recreation. Different calcium phosphate phases have been accepted as scaffold’s components with positive influence on osteoinduction and differentiation of human mesenchymal stem cells, in terms of their higher fraction within the graft. Nevertheless, the creation of unlimited nutrients diffusion through newly formed grafts is of great importance. The media flow accomplished by perfusion forces can provide physicochemical, and also, biomechanical stimuli for three-dimensional bone-construct growth. In the present study, the influence of a different scaffold’s composition on the human mesenchymal stem cells (hMSCs) differentiation performed in a U-CUP bioreactor under perfusion conditioning was investigated. The histological and immunohistochemical analysis of cultured bony tissues, and the evaluation of osteogenic genes’ expression indicate that the lower fraction of in situ formed hydroxyapatite in the range of 10–30% within chitosan scaffold could be preferable for bone-construct development.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Kemijsko inženjerstvo, Temeljne tehničke znanosti

Napomena
Glavni autori: Anamarija Rogina i Marica Ivanković

POVEZANOST RADA