Pregled bibliografske jedinice broj: 888644
An elution mobile phase and process for immunoaffinity chromatography of viruses
An elution mobile phase and process for immunoaffinity chromatography of viruses
Hrvatski patentni glasnik 16 (2017) 1.
CROSBI ID: 888644 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
An elution mobile phase and process for immunoaffinity chromatography of viruses
Autori
Brgles, Marija ; Halassy, Beata ; Forčić, Dubravko
Broj patenta
WO 2017/129450 A1
Godina
2017
Nositelj prava
Sveučilište u Zagrebu, Zagreb, Hrvatska
Sažetak
The present invention discloses a novel composition of the elution mobile phase for virus purification by immunoaffinity chromatography which consists of one or more amino acids: L-serine, L-asparagine, or L- glutamine, or their salts with pharmaceutically acceptable acids ; one or more auxiliary ingredients: L-arginine, glycine or imidazole, or their salts with pharmaceutically acceptable acids ; one or more pharmaceutically acceptable pH adjusting agents for correcting the pH value of the mobile phase from pH = 6.0 - 8.0 ; and purified water, up to 100 % w/w of the mobile phase composition. The invention provides the use of immunoaffinity chromatography as a key step in the production of viral vaccines and/or viral vectors, in separation of infectious from non-infectious viral particles, and for enrichment of the viral suspension in infectious viral particles.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biotehnologija
POVEZANOST RADA
Projekti:
HRZZ-UIP-2013-11-8193 - Kromatografsko pročišćavanje biomolekula i njihova karakterizacija (CHROBIO) (Brgles, Marija, HRZZ - 2013-11) ( CroRIS)
Ustanove:
Sveučilište u Zagrebu