Naslov
Spatial mapping of whisk fern specialized metabolites using MALDI Imaging

Autori
Šamec, Dunja ; Lange, Bernd Markus

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
4th Meeting on Biotechnology of Plant Products- Green for Good IV : Programme and Abstracts / - Olomouc : EFB Regional Branch Office C.R. Hana, Phytochemical Society of Europe, European Federation of Biotechnology, 2017, 50-50

Skup
Biotechnology of Plant Productes- Green for Good IV

Mjesto i datum
Olomouc, Češka Republika, 19.06.2017. - 22.06.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MALDI Imaging ; whisk fern

Sažetak
Phytochemicals are specialized plant metabolites which play important roles in the areas of plant biochemistry, pharmacy and medical science because of their significant bioactivities. Mass spectrometric analyses in combination with separation techniques can provide information about a particular compound which is present in the sample although the information about its distribution in the sample is lost in the extraction process. Newer technologies using Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry (IMS) allows investigation of the small molecules distribution within biological systems through the direct analysis of thin tissue sections. Technique involves laser desorption ionization with prior application of a chemical matrix. The sample is introduced into a loading chamber and is rastered with respect to a laser, while mass spectra are recorded at each position across the sample. The signal intensity for an ion can then be plotted versus the XY position on the specimen, thereby enabling the generation of an ‘ion map’ or mass spectral image. IMS does not involve any form of separation prior to ionization, so the resulting data are often highly complex due to the level of detail observed and the potential for background interferences. In our study, using combination of MALDI imaging with ion mobility-mass spectrometry we successfully identified and localized predominant metabolites, biflavonoid amentoflavone and signature phenolic glycoside psilotin and 3'-hydroxypsilotin in whisk fern Psilotum nudum (L.) P. Beauv.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA