Naslov
Solvent extraction and chromatographic determination of polyphenols in olive pomace

Autori
Vitali Čepo, Dubravka ; Albahari, Petra ; Zovko Končić, Marijana ; Radić, Kristina ; Jurmanović, Sanja ; Jug, Mario

Izvornik
Hrana u zdravlju i bolesti (2233-1220) 6 (2017), 1; 7-14

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
olive pomace ; extraction ; HPLC-DAD ; hydroxytyrosol ; tyrosol ; oleuropein
(olive pomace ; extraction ; HPLC-DAD ; hydroxytyrosol ; tyrosol, oleuropein)

Sažetak
Olive oil mill waste obtained after two-phase olive oil extraction process was subjected to conventional liquid solvent extraction under different pH/temperature/ duration conditions using different types of food-grade solvents. The independent variables were: solvent type (ethanol percentage), extraction temperature (20-90 °C), extraction time (30 min-24 h) and pH (2-10.3) of extraction solvent while the response variables were total phenolic content and antioxidant activity of obtained extracts. The optimum solvent extraction conditions for phenols were 120 min at 70 °C using 60% ethanol as extraction solvent, at solvent to sample ratio 5:1 (v/w). For quantification of major bioactive olive polyphenols hydroxytyrosol, tyrosol and oleuropein in obtained extracts, fast and simple RP-HPLC-DAD method was developed and validated. Oleuropein was presented in highest amounts with average value of 115.14±0.19 mg/kg of fresh olive pomace.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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