Naslov
Cross-reactivity of serum indices on Abbott Architect c8000, Roche Cobas 6000 and Beckman Coulter AU5800 analyzers: HIL interference project of the Working group for the preanalytical phase, Croatian Society of Medical Biochemistry and Laboratory Medicine

Autori
Miler, Marijana ; Nikolac, Nora ; Ćelap, Ivana ; Filipi, Petra ; Hemar, Marina ; Kocijančić, Marija ; Šimundić, Ana-Maria ; Šupak Smolčić, Vesna ; Vrtarić, Alen

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Chemistry and Laboratory Medicine / Plebani, Mario - Berlin : Walter de Gruyter, 2017, EA20-eA20

Skup
4th EFLM-BD European Conference on Preanalytical Phase

Mjesto i datum
Amsterdam, Nizozemska, 24.03.2017. - 25.03.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cross-reactivity, hemolysis, icteria, interference, lipemia, serum indices

Sažetak
Serum indices, hemolysis (H), icteria (I), lipemia (L) are determined automatically on chemistry analyzers. HIL absorbance spectra patterns overlap which could result with HIL cross-reactivity when multiple interferences are present. We aimed to investigate interference cross-reactivity and to compare cross-reactivity of indices between three different biochemistry analyzers. Study included Abbott Architect c8000, Beckman Coulter AU5800 (BC) and Roche Cobas 6000. For each interference, 6 increasing levels of interfering substances were added to serum pool (hemolysis: 0.3-10 g/L hemoglobin (Hb) by needle aspiration, icteria: 35-675 µmol/L of dissolved bilirubin, lipemia: 0.1-1.4 g/L of Intralipid). HIL indices were measured in duplicate before and after addition of second interferent (approximate values H=5 g/L, I=400 µmol/L, L=1 g/L of Intralipid) and biases between those measurements were calculated. BC provides semiquantitative results (5 categories), while Abbott and Roche express indices as corresponding concentrations: hemoglobin (g/L) for H, bilirubin (μmol/L) for I and Intralipid (g/L) for L index. Less than 10% was considered acceptable bias for Abbott and Roche, and for BC agreement of result category. Hemolysis decreased I index on Abbott and Roche (for 20% and 14%, respectively). On BC, hemolysis increased I index. L index on Abbott was higher (for 35-180%), while on Roche and BC, L index was lower (11-32% for Roche) after adding free Hb. Adding bilirubin had no effect on H index on Abbott, while on Roche and BC, H index was higher (13-35%). Bilirubin also changed L index for more than 30% on all analyzers. I index on Abbott and Roche was lower after adding Intralipid (mean bias 75% and 28%, respectively). Lipemia interfered with H index on all three analyzers (for approximately 14%). Almost all tested interference combinations showed cross- reactivity. Serum indices could be erroneous if more than one interfering substances is present in serum sample.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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