Naslov
Pentatrichomonas hominis COINFECTION IN A PUPPY FROM A SLOVENIAN ANIMAL SHELTER

Autori
Brložnik, Maja ; Faraguna, Siniša ; Slavec, Brigita ; Kostanjšek, Rok ; Vergles Rataj, Aleksandra ; Gruntar, Igor

Izvornik
Slovenian veterinary research (1580-4003) 53 (2016); 229-235

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Pentatrichomonas hominis ; dog ; isolation ; cultivation ; PCR ; DNA sequencing ; scanning electron microscopy

Sažetak
A 3-month-old dog originating from a Slovenian animal shelter presented with acute bloody, soft, foamy and malodorous diarrhoea. The clinical examination, haematology and serum biochemistry were unremarkable. Ultrasonography of the abdomen showed prominent mesenteric lymph nodes and the presence of echogenic content within the small intestine. Light microscopy of a native smear and a wet mount darkfield microscopy examination of the faecal material showed motile trichomonad-like organisms with a particular circular motion. The flotation and SAF (Sodium acetate - acetic acid - formalin solution) method using light microscopy revealed eggs of nematode Toxocara canis and protozoan oocysts of Isospora spp. Trichomonad-like organisms were successfully isolated and cultivated in axenic culture. Light microscopy of Giemsa-stained trichomonads showed the presence of five flagella, and Pentatrichomonas hominis (P. hominis) was presumptively diagnosed. The diagnosis was confirmed by the Polymerase Chain Reaction (PCR) followed by DNA sequencing and the Scanning Electron Microscopy (SEM) of cultured trichomonad isolates. The PCR and sequencing results confirmed a 99% homology of the P. hominis isolates with isolates from other studies, originating both from humans and animals, which suggests that P. hominis could have zoonotic potential and have been transmitted from animals to people via the per- oral route. This is also the first report on P. hominis involvement in clinical diseases in dogs in Slovenia.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA