Naslov
Double-strand break repair mechanisms in Escherichia coli: Recent insights

Autori
Đermić, Damir

Izvornik
Advances in genomics and genetics (1179-9870) 5 (2015); 35-42

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
RecA nucleoprotein filament ; homologous recombination ; exonucleases ; genome stability ; 3´-overhang metabolism

Sažetak
In order to survive, all organisms must repair the continuous appearance of double-strand breaks (DSBs) in their DNA. Escherichia coli does this by RecA-dependent homologous recombination (HR), during which the RecA protein is assembled on a 3´-terminated overhang that is created by a process called DNA end resection. The RecA nucleoprotein filament searches for and invades an intact homologous DNA sequence, creating a central HR intermediate. This review describes recent insights into HR and DSB repair in E. coli, especially the processes that precede the formation of a RecA nucleoprotein filament, with an emphasis on the regulation of 3´-tail metabolism. Since HR is a highly conserved process, the parallels to DSB repair in eukaryotic systems are discussed, bearing in mind that the lessons learned from studies in simpler bacterial models may be useful for studying DSB repair and the maintenance of genome stability in eukaryotes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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