Pregled bibliografske jedinice broj: 880269
Characterization of the ribosomal RNA operon rrnF from Streptomyces rimosus
Characterization of the ribosomal RNA operon rrnF from Streptomyces rimosus // 9th Regional Meeting of Biochemists, Biophysicists and Biotechnologists : abstracts / Vitale, Ljubinka (ur.).
Zagreb: Croatian Biochemical Society, 1990. str. 48-48 (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 880269 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Characterization of the ribosomal RNA operon rrnF from Streptomyces rimosus
Autori
Plohl, Miroslav ; Pujić, Petar ; Durajlija, Sonja ; Gamulin, Vera
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
9th Regional Meeting of Biochemists, Biophysicists and Biotechnologists : abstracts
/ Vitale, Ljubinka - Zagreb : Croatian Biochemical Society, 1990, 48-48
Skup
Regional Meeting of Biochemists, Biophysicists and Biotechnologist (9 ; 1990)
Mjesto i datum
Opatija, Hrvatska, 28.03.1990. - 30.03.1990
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
rRNA genes, S.rimosus, DNA sequencing, rho idependent terminator
Sažetak
Restriction sites for BamH1 endonuclease do not exist in S.rimosus rRNA operons. Digestion of S.rimosus genomic DNA with BamH1 leaves sic operons (A-F) on six DNA fragments. The smallest fragment is 8.8 kb long and contains rRNA operon named rrnaF..We cloned this operon into pBR322 and characterisation of its primary structure is in progress. Over two thousand nucleotides are already sequenced partially covering16S and 23S rRNA genes and whole 5S rRNA gene (116 nucleotides). Genes are organized in the order 16S-23S-5S. 5S rRNA gene is followed by two long inverted repeats, structure characteristic for rho independent terminator. tRNA genes are not located at the 3'end of rrnaF operon. Two very conserved Pst1 sites 4.5 kb apart, foud in all six rRNA operons, are located around position 465 in 16S RNA gene and position 85 in 5S RNA gene, which means that in all rRNA operons spacers between genes are short and of the similar size.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Ustanove:
Institut "Ruđer Bošković", Zagreb
Profili:
Sonja Durajlija-Žinić
(autor)
Petar Pujić
(autor)
Vera Gamulin
(autor)
Miroslav Plohl
(autor)