Pregled bibliografske jedinice broj: 879746
Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli
Construction of two stable bifunctional plasmids for Streptomyces spp. and Escherichia coli // FEMS microbiology letters, 83 (1991), 3; 317-321 doi:10.1111/j.1574-6968.1991.tb04483.x (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 879746 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Construction of two stable bifunctional plasmids for
Streptomyces spp. and Escherichia coli
Autori
Durajlija, Sonja ; Pigac, Jasenka ; Gamulin, Vera
Izvornik
FEMS microbiology letters (0378-1097) 83
(1991), 3;
317-321
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
Shuttle vectors ; Plasmid stability ; Multiple cloning sites ; Streptomyces rimosus ; Streptomyces lividans ; Transfer RNA genes
Sažetak
Two bifunctional plasmid vectors pZG5 (7.45 kb) and pZG6 (6.95 kb), for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of the multicopy broad-host- range Streptomyces plasmid pIJ350 with E. coli plasmids Bluescribe M13− (pZG5) or pUC18 (pZG6). Both plasmids possess several unique restriction sites suitable for DNA cloning. Stable transformants of Streptomyces rimosus R6 and S. lividans 66 were obtained, harboring intact plasmids regardless of colony age or multiple subculturing. Moreover, pZG5 and pZG6 were successfully used to introduce several homologous transfer RNA genes into S. rimosus.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Ustanove:
Pliva-Istraživački institut,
Institut "Ruđer Bošković", Zagreb
Citiraj ovu publikaciju:
Časopis indeksira:
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Scopus
- MEDLINE
Uključenost u ostale bibliografske baze podataka::
- Index Medicus
- PubMed
