Naslov
Qualitative HPLC-DAD-ESI-TOF-MS analysis, cytotoxic and apoptotic effects of Croatian endemic Centaurea ragusina L. aqueous extracts

Autori
Radan, Mila ; Carev, Ivana ; Tešević, Vele ; Politeo, Olivera ; Čikeš Čulić, Vedrana

Izvornik
Chemistry & biodiversity (1612-1872) 14 (2017), 9; E1700099, 9

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Centaurea ragusina ; cytotoxic ; apoptotic ; MTT ; sesquiterpene lactone

Sažetak
Centaurea ragusina L., an endemic Croatian plant species, revealed a good cytotoxic activity of aqueous extracts on human bladder (T24) and human glioblastoma (A1235) cancer cell lines. The chemical constituents were tentatively identified using high performance liquid chromatography HPLC-DAD-ESI-TOF-MS in negative ionization mode. The main compounds of herba extract were sesquiterpene lactones: solstitialin A-3, 13 diacetate and epoxyrepdiolide ; organic acid: quinic acid. The main compounds of flower extract were organic acids: quinic acid, citric acid and malic acid ; sesquiterpene lactone: cynaropicrin ; phenolic compounds: chlorogenic acid and phenylpropanoid: syringin. The aqueous extracts of C. ragusina were investigated for correlation of their effects on human bladder (T24) and human glioblastoma (A1235) cancer cell lines using the MTT assay. Although both extracts showed significant dose- and time-dependent cytotoxicactivity against both cancer cell lines, the flower extract exhibited slightly higher activity. In order to determine type of cell death induced by treatment, cell lines were exposed subsequently to a treatment with both flower and herba aqueous extracts. The majority of the cells died by induced apoptosis treatment. Flower aqueous extract (26.25 %), compared to a leaf aqueous extract (22.15 %) showed slightly higher percentage of an apoptosis in T24 cells, when compared to a non-treated cells (0.04 %).

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA