Naslov
Detection of Chlamydiaceae in different species of chelonians

Autori
Lukač, Maja ; Gottstein, Željko ; Prukner-Radovčić, Estella ; Quilicot, Ana Marquiza ; Laroucau, Karine ; Azziz, Rachid ; Nedeljković, Gordana ; Horvatek Tomić, Danijela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of Zoo and Wildlife Health Conference 2017 / Czirjak, G. A. ; Prugel, J - Berlin : Leibniz Institute for Zoo and Wildlife Research, 2017

Skup
Zoo and Wildlife Health Conference 2017

Mjesto i datum
Berlin, Njemačka, 24.05.2017. - 27.05.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Chlamydiaceae ; chelonians, qPCR

Sažetak
Chlamydial species are increasingly recognized as infective and potentially pathogenic organisms in reptiles. Different types of symptoms were described in reptiles with Chlamydia sp. infections, usually with inflammatory lesions mostly in the spleen, heart, lungs and liver. Chlamydia can be isolated from clinically asymptomatic reptiles as well. To check for the presence of Chlamydia, 51 choanal and cloacal swabs were taken from different free-living and captive chelonians, 30 red eared sliders (Trachemys scripta elegans), 6 marginated tortoises (Testudo marginata) and 15 loggerhead sea turtles (Caretta caretta) during the routine health check up. The DNA was extracted by using QIAmp DNA Mini Kit (QIAGEN, Germany) and examined by Chlamydiaceae-specific Real-time PCR as described by EHRICHT et al. (2006). Positive samples were further examined by species specific Real-time PCR. Partial 16S rRNA genes were further sequenced. In total, 26 samples were found positive by Chlamydiaceae-specific Real-time PCR: 17 from red eared sliders, six from loggerhead sea turtles and three from marginated tortoises. Out of them, two samples from read eared slider were found positive for C. psittaci by species specific RT-PCR. Partial 16S rRNA gene sequencing revealed the similarity of one sample to the family Waddliaceae (96% identity) and one sample to the family Simkaniaceae (93% identity). Both of these samples were from loggerhead sea turtles. The role of chlamydial species in reptiles is still unclear, so that further investigation of uncharacterised Chlamydia and their impact to both reptile and human health is needed.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina

POVEZANOST RADA