Naslov
PRIMJENA MIKRONUKLEUS TESTA NA ERITROCITIMA MIŠA U PROCJENI RADIOPROTEKTIVNIH UČINAKA PROPOLISA I NJEGOVIH POLIFENOLNIH SASTAVNICA
(ASSESSMENT OF THE RADIOPROTECTIVE EFFECTS OF PROPOLIS AND ITS POLYPHENOLIC COMPOUNDS IN MICE USING MICRONUCLEUS TEST IN RETICULOCYTES)

Autori
Benković, Vesna ; Horvat Knežević, Anica ; Cikač, Tihana ; Milić, Mirta ; Kopjar, Nevenka

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), ostalo

Izvornik
Proceedings of the Eleventh Symposium of the Croatian Radiation Protection Association / Radolić, Vanja ; Poje Sovilj, Marina ; Krajcar Bronić, Ines - Zagreb : Studio HS internet, 2017, 167-172

Skup
Eleventh Symposium of the Croatian Radiation Protection Association

Mjesto i datum
Osijek, Hrvatska, 04.04.2017. - 07.04.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
propolis ; miševi soja Swiss albino ; zaštita od djelovanja ionizirajućeg zračenja ; mikronukleus test ; kvercetin ; kafeinska kiselina ; krizin ; naringin ; retikulociti
(propolis ; Swiss albino mice ; radioprotection ; ionising radiation ; micronucleus assay ; quercetin ; caffeic acid ; chrysin ; naringin ; reticulocytes)

Sažetak
Hematopoietic stem cells of mice are extremely sensitive to the effects of ionizing radiation. Genome damage lead to formation of micronuclei (MN) that remain in the cytoplasm, in contrast to the nucleus, which during maturation of erythrocytes is expelled from the cell. Micronuclei can be detected by sensitive staining methods using dyes which specifically bind to DNA. This principle is used in the so called in vivo MN test. The aim of this study was to evaluate the radioprotective effects of propolis and its polyphenolic compounds: quercetin, caffeic acid, chrysin and naringin on the level of genome damage in Swiss albino mice. The mice were treated for three days with the test substances (100 mg/kg, i.p.) and then radiated - irradiation at a dose 4 Gy. Non-irradiated animals were studied simultaneously. 48 h after irradiation blood samples were collected and blood smears were prepared. Following staining with acridine-orange, preparations were analysed under fluorescent microscope. In each sample 2000 reticulocytes were counted, and the total number and distribution of MN was determined. Groups of mice that were pre-treated with propolis and flavonoids had a smaller number of MN compared to the irradiated mice. Particularly effective radioprotector was quercetin. Test substances did not cause significant damage to the genome of non- irradiated mice. The obtained results speak in favour of further research of natural compounds with radioprotective properties, especially to clarify the mechanisms of their protective effect on DNA, and to establish their potential use as food supplements in patients who undergo radiotherapy or in subjects occupationally exposed to ionising radiation.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA