Naslov
Adjuvant activity of peptidoglycan monomer and its metabolic products

Autori
Halassy, Beata ; Krstanović, Marina ; Frkanec, Ruža ; Tomašić, Jelka

Izvornik
Vaccine (0264-410X) 21 (2003), 9/10; 971-976

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
peptidoglycan monomer; adjuvants; peptidoglycan metabolites

Sažetak
Peptidoglycan monomer (PGM) is a natural compound of bacterial origin. It is a non-toxic, non-pyrogenic, water-soluble immunostimulator potentiating humoral immune response to ovalbumin (OVA) in mice. It is fast degraded and its metabolic products - the pentapeptide (PP) and the disaccharide (DS) - are excreted from the mammalian organism upon parenteral administration. Present study investigates a) whether PGM could influence the long living memory generation ; b) whether metabolic products retain adjuvant properties of the parent compound and contribute to its adjuvanticity. We report now that mice immunised twice with OVA+PGM had significantly higher anti-OVA IgG levels upon challenge with antigen alone six months later in comparison to control group immunised with OVA only. PP and DS were prepared enzymatically in vitro as apyrogenic and chemically pure compounds. When mice were immunised with OVA plus PP and DS, respectively, the level of anti-OVA IgGs in sera was not higher than in mice immunised with OVA alone, while PGM raised the level of specific antibodies. Results implicate that the adjuvant active molecule, capable of enhancing long-living memory generation, is PGM itself, and none of its metabolic products

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

Napomena
S.I.: Immunopotentiators in Modern Vaccines.

POVEZANOST RADA