Naslov
Detoxification of aflatoxin B1 and ochratoxin A by gamma radiation

Autori
Domijan, Ana-Marija ; Marjanović, Ana Marija ; Vulić, Ana ; Tartaro Bujak, Ivana ; Pavičić, Ivan ; Pleadin, Jelka ; Markov, Ksenija ; Mihaljević, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
International Conference on Applications of Radiation Science and Technology (ICARST 2017) : abstracts / - Beč : International Atomic Energy Agency (IAEA), 2017, 335-335

Skup
International Conference on Applications of Radiation Science and Technology

Mjesto i datum
Beč, Austrija, 24.04.2017. - 28.04.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Aflatoxin B1, Ochratoxin A, gamma-radiation

Sažetak
Mycotoxins aflatoxin B1(AFB1) and ochratoxin A(OTA)are widely distributed food contaminants that have adverse effects to animal and human health. In order to reduce mycotoxin contamination and protect animal and human health, various strategies are applied. Some studies indicate that γ-irradiation is effective in reducing mycotoxin contamination. Therefore the aim of this study was to investigate the effect of γ-irradiation on the AFB1 and OTA degradation as well as toxicity of mycotoxin radiolytic products in vitro. Mycotoxins stock solutions in methanol (50 mM) were γ-irradiated with doses of 5 and 10 kGy and dose rate of 140 Gy/min using panoramic 60Co source in the Radiation Chemistry and Dosimetry Laboratory at the Ruđer Bošković Institute. The dose rate was established using the ethanol-chlorobenzene dosimetry system. Molecule structure analysis of non-irradiated and irradiated AFB1 and OTA was performed by liquid chromatography tandem mass spectrometry(HPLC-MS/MS).Toxicity of non- irradiated and irradiated AFB1 and OTA(in concentration 1–500 µM ; 24 h) was tested on HepG2, SH-SY5Y and Pk15 cells by quantitative colourimetric MTTassay. AFB1andOTAmoleculeswereeffectivelydegradedevenat 5 kGy of γ-irradiation. The signal intensity for non-irradiated AFB1 was 16 times higher than for irradiated AFB1 (5 kGy)and signal intensity for non-irradiated OTA was twice higher than irradiated OTA (5kGy). These results indicate that AFB1 has greater susceptibility than OTA to γ-irradiation. Besides of fragment ions of AFB1 or OTA with a mass less then the parent ion, several radiolytic products with mass higher then the parent ion were detected. These results strongly indicate the contribution of addition reactions caused by free radicals generated in solution during γ-radiolysis. Results on cytotoxicity indicate that radiolytic product of irradiated AFB1 and OTA are less toxic to HepG2, SH-SY5Y and Pk15 cell lines than non- irradiated mycotoxins(parent compounds). Based on the AFB1 and OTA structure modifications induced by γ-irradiation, free radical mechanisms are operative in the irradiation of mycotoxins. Additionally, cell viability assay demonstrated that mycotoxins radiolytic products are less toxic to cells than parent nonirradiated compound. Based on these results γ-irradiation can be considered as an effective method for the detoxification of mycotoxins.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA