Naslov
The effect of adjuvants on immune reaction to snake venoms

Autori
Lang Balija, Maja ; Halassy Špoljar, Beata ; Dojnović, Biserka ; Vrdoljak, Anto ; Tomašić, Jelka ; Vranešić, Branka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Third World Congress on Vaccines and Immunisation / Kurstak Edouard - : Infections Control World Organisation, 2002, 84-84

Skup
Third World Congress on Vaccines and Immunisation

Mjesto i datum
Opatija, Hrvatska, 04.06.2002. - 09.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Sažetak
Long-nosed viper (V. ammodytes) is one of the most poisonous snakes, that is widely distributed through central Europe. Specific antiserum, that is produced by gradual immunisation of big animals (horse, donkey, sheep), is the only available therapeutic drug for curing snake venom poisoning. The aim of our work was to establish a mice model for studying immune reaction (IR) specific for the snake venom, that is a toxin and an immunogen at the same time, and to monitor the influence of experimental adjuvants, peptidoglycan monomer GlcNAc-MurNAc-L-Ala-D-isoGln-mesoDAP(wNH2)-D-Ala-D-Ala (PGM) and L-(adamant-2-il)-Gly-L-Ala-D-isoGln (AdTP2), on this reaction. Optimal immunisation protocol was found by varying the amount of the venom dose and the schedule of immunisation. The amount of the antigen was scaled down starting with the maximal quantity of the venom that is not subcutaneously lethal for mice. The immunisation schedule, we found to be optimal, included four s.c. immunisations in three week intervals. The specific IR was monitored by ELISA measuring venom-specific IgGs (qualitatively and quantitatively). The selected sera with high titres of specific antibodies were tested for their ability to neutralise snake venom. The stimulatory effect of adjuvants, PGM and AdTP2, was demonstrated using suboptimal doses of venom.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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