Pregled bibliografske jedinice broj: 87407
Adjuvant effect of peptidoglycan monomer in immune reaction specific for synthetic peptides of measles virus origin
Adjuvant effect of peptidoglycan monomer in immune reaction specific for synthetic peptides of measles virus origin // Third World Congress on Vaccines and Immunisation / Kurstak, Edouard (ur.).
Opatija, Hrvatska: Infections Control World Organisation, 2002. str. 82-83 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 87407 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Adjuvant effect of peptidoglycan monomer in immune reaction specific for synthetic peptides of measles virus origin
Autori
Mateljak, Sanja ; Halassy Špoljar, Beata ; Dojnović, Biserka ; Bouche, F. Fabienne ; Putz, M. Mike ; Muller, P. Claude ; Tomašić, Jelka
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Third World Congress on Vaccines and Immunisation
/ Kurstak, Edouard - : Infections Control World Organisation, 2002, 82-83
Skup
Third World Congress on Vaccines and Immunisation
Mjesto i datum
Opatija, Hrvatska, 04.06.2002. - 09.06.2002
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Sažetak
Synthetic peptides comprising critical epitopes from the measles virus (MV) have a great potential as vaccines1. Peptidoglycan monomer (PGM) is a disaccharide pentapeptide originating from the cell wall peptidoglycan of Brevibacterium divaricatum. It is apyrogenic, non-toxic and water-soluble compound possessing adjuvant properties2. The aim of this work was to determine the effect of PGM on immune reaction to five chimeric peptides comprising B cell epitope from MV hemagglutinin and different immunodominant T cell epitopes from MV fusion protein or nucleoprotein. The peptides differ in length (30-45 amino acids) depending whether they have one or two copies of either T cell or B cell epitope. Mice were immunised subcutaneously with individual peptides (50 or 100 mg) alone and in combination with PGM (200 or 400 mg). Peptides- or B cell epitope-specific antibodies were determined by ELISA after two boosters (two weeks intervals). Anti-peptide antibody responses were different for each peptide. PGM increased the level of specific antibodies to four out of five tested peptides. All immunised mice sera exhibited strong crossreactivity with all tested chimeric peptides and B cell epitope peptide. However, none of the sera induced could neutralise measles virus (Edmonston-Zagreb vaccine strain) in vitro. (1el Kasmi, K.C. et al. Vaccine 17(1999)2436 ; Tomašić, J. et al. Vaccine 18(2000)1236).
Izvorni jezik
Engleski
Znanstvena područja
Kliničke medicinske znanosti
