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Pregled bibliografske jedinice broj: 873240

Investigation of mumps and measles virus proteome and lipidome


Sviben, Dora; Forčić, Dubravko; Halassy, Beata; Allmaier, Günter; Marchetti-Deschmann, Martina; Brgles, Marija
Investigation of mumps and measles virus proteome and lipidome // 25. hrvatski skup kemičara i kemijskih inženjera s međunarodnim sudjelovanjem : knjiga sažetaka / Šantić, Ana ; Đaković, Marijana (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2017. str. 85-85 (predavanje, domaća recenzija, sažetak, znanstveni)


CROSBI ID: 873240 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Investigation of mumps and measles virus proteome and lipidome

Autori
Sviben, Dora ; Forčić, Dubravko ; Halassy, Beata ; Allmaier, Günter ; Marchetti-Deschmann, Martina ; Brgles, Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
25. hrvatski skup kemičara i kemijskih inženjera s međunarodnim sudjelovanjem : knjiga sažetaka / Šantić, Ana ; Đaković, Marijana - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2017, 85-85

Skup
Hrvatski skup kemičara i kemijskih inženjera s međunarodnim sudjelovanjem(25 ; 2017)

Mjesto i datum
Poreč, Hrvatska, 19.04.2017. - 22.04.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
mumps virus, measles virus, mass spectrometry, MALDI-TOF/TOF

Sažetak
Measles (MEV) and mumps (MUV) virus are enveloped, nonsegmented, negative single stranded RNA viruses of the family Paramyxoviridae [1]. They are the cause of mumps and measles, respectively, both of which can be prevented by vaccination. Lipid bilayer of enveloped viruses is not directly encoded by the virus but acquired from the host cell membranes. Lipid composition of some viruses was found to resemble that of host cell plasma membrane, but in other cases significant differences were noticed [2, 3]. Apart from virus-coded proteins, it has been established that various enveloped viruses incorporate also host cell proteins (HCPs) both inside the viral particle and in the lipid bilayer. Indications exist that some HCPs are specifically taken up by the viral particles by direct interactions with viral proteins, but HCPs might also be included by non-specific interactions, or as contamination arising from the presence of exosomes in virus preparations [4-6]. The aim of our research was investigation of MEV and MUV lipidome and proteome, consisting of 8 and 9 virus-coded proteins, respectively, and detection of HCPs potentially present in virus preparations. Viruses were grown in Vero cells, microfiltrated, purified by hydrophobic interaction chromatography (HIC) and eluates concentrated by ultracentrifugation. Alternatively, viruses were purified only by ultracentrifugation. Also, supernatants from non-infected Vero cells were purified and analyzed as described for virus samples. Obtained samples were resolved by SDS-PAGE, protein bands excised and in-gel digestion by trypsin was performed. Lipids were extracted from samples by Bligh-Dyer method and separated using 2D-HPTLC. Lipids and proteins were identified by MALDI-TOF/TOF-MS. Results implicate that there are no considerable differences between lipid composition of viral particles and Vero cells. All viral structural proteins were detected in viral samples, including fusion protein both for MUV and MEV which was not previously found. Also, various HCPs previously reported in other purified virus preparations such as actin, cyclophilin A, and annexin A2 were detected. References [1] C.L. Afonso, G.K. Amarasinghe, K. Banyai, Y. Bao et al., Arch. Virol. 161 (2016) 2351- 2360. [2] M. Brgles, M. Bonta, M. Šantak, M. Jagušić, D. Forčić, B. Halassy, G. Allmaier and M. Marchetti-Deschmann, Virology Journal 13 (2016) DOI:10.1186/s12985-016-0463-0. [3] B. Brügger, B. Glass, P. Haberkant, I. Leibrecht, F.T. Wieland and H.-G. Kräusslich, Proc. Natl. Acad. Sci. U.S.A. 103 (2006) 2641- 2646. [4] R. Cantin, S. Méthot and M.J. Tremblay, J. Virol. 79 (2005) 6577-6587. [5] E.K. Franke, H.E. Yuan and J. Luban, Nature 372 (1994) 359-362. [6] E. Nolte-‘t Hoen, T. Cremer, R.C. Gallo and L.B. Margolis, Proc. Natl. Acad. Sci. U.S.A. 113 (2016) 9155-9161.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekti:
HRZZ-UIP-2013-11-8193 - Kromatografsko pročišćavanje biomolekula i njihova karakterizacija (CHROBIO) (Brgles, Marija, HRZZ - 2013-11) ( CroRIS)

Ustanove:
Sveučilište u Zagrebu

Profili:

Avatar Url Marija Brgles (autor)

Avatar Url Beata Halassy (autor)

Avatar Url Dubravko Forčić (autor)


Citiraj ovu publikaciju:

Sviben, Dora; Forčić, Dubravko; Halassy, Beata; Allmaier, Günter; Marchetti-Deschmann, Martina; Brgles, Marija
Investigation of mumps and measles virus proteome and lipidome // 25. hrvatski skup kemičara i kemijskih inženjera s međunarodnim sudjelovanjem : knjiga sažetaka / Šantić, Ana ; Đaković, Marijana (ur.).
Zagreb: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo, 2017. str. 85-85 (predavanje, domaća recenzija, sažetak, znanstveni)
Sviben, D., Forčić, D., Halassy, B., Allmaier, G., Marchetti-Deschmann, M. & Brgles, M. (2017) Investigation of mumps and measles virus proteome and lipidome. U: Šantić, A. & Đaković, M. (ur.)25. hrvatski skup kemičara i kemijskih inženjera s međunarodnim sudjelovanjem : knjiga sažetaka.
@article{article, author = {Sviben, Dora and For\v{c}i\'{c}, Dubravko and Halassy, Beata and Allmaier, G\"{u}nter and Marchetti-Deschmann, Martina and Brgles, Marija}, year = {2017}, pages = {85-85}, keywords = {mumps virus, measles virus, mass spectrometry, MALDI-TOF/TOF}, title = {Investigation of mumps and measles virus proteome and lipidome}, keyword = {mumps virus, measles virus, mass spectrometry, MALDI-TOF/TOF}, publisher = {Hrvatsko dru\v{s}tvo kemijskih in\v{z}enjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo}, publisherplace = {Pore\v{c}, Hrvatska} }
@article{article, author = {Sviben, Dora and For\v{c}i\'{c}, Dubravko and Halassy, Beata and Allmaier, G\"{u}nter and Marchetti-Deschmann, Martina and Brgles, Marija}, year = {2017}, pages = {85-85}, keywords = {mumps virus, measles virus, mass spectrometry, MALDI-TOF/TOF}, title = {Investigation of mumps and measles virus proteome and lipidome}, keyword = {mumps virus, measles virus, mass spectrometry, MALDI-TOF/TOF}, publisher = {Hrvatsko dru\v{s}tvo kemijskih in\v{z}enjera i tehnologa (HDKI) ; Hrvatsko kemijsko drustvo}, publisherplace = {Pore\v{c}, Hrvatska} }




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