Naslov
Hospital wastewater as a route for transmission of carbapenem-resistant Acinetobacter baumannii outside the hospital setting

Autori
Goić Barišić, Ivana ; Kovačić, Ana ; Tonkić , Marija ; Šeruga Musić, Martina ; Novak, Anita ; Rubić, Žana ; Hrenović, Jasna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27th European Congress of Clinical Microbiology and Infectious Diseases / - Beč, 2017, EV012-EV012

Skup
27th European Congress of Clinical Microbiology and Infectious Diseases

Mjesto i datum
Beč, Austrija, 22.04.2017. - 25.04.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Acinetobacter baumannii, hospital, wastewater

Sažetak
Background: Among the „ESCAPE“ pathogens Acinetobacter baumannii is the most frequently encountered microorganism in the hospital setting causing serious infections, especially in the intensive care units. As a leading nosocomial pathogen of the 21st century and having the ability to acquire resistance to almost all antimicrobial agents, origin and epidemiology of this emerging hospital pathogen is under a great concern worldwide. Material/methods: For prospective investigation hospital wastewater was sampled for five times, on two different locations, in the period of October 2015 until April 2016 in University Hospital Centre Split, the second largest hospital in Croatia. Samples were taken in 500 ml sterile bottles and inoculated within two hours on solid media. The isolation of A. baumannii was performed on CHROMagar Acinetobacter after incubation at 42°C/48h. Molecular identification was performed by amplification, sequencing and phylogenetic analyses of rpoB gene. The presence of blaOXA genes encoding OXA-type carbapenemases (OXA-51-like, OXA-23like, OXA-40-like, and OXA-58-like) was investigated by multiplex PCR and sequencing. Genotyping was performed using PFGE analysis and the results were compared with unpublished data of previously typed clinical isolates from the same monitoring period. Results: During monitoring period fourteen carbapenem and also multi-resistant isolates of A. baumannii were isolated from hospital wastewater. Phylogenetic analyses showed close association of oxacillinases (OXA-51-like and OXA-40-like) in Acinetobacter isolates originated from hospital wastewater to those from clinical isolates in the same hospital. Conclusions: With this study we confirmed the possibility of the spread of multi-resistant A. baumannii trough hospital wastewater in nature. The possible impact on the horizontal transfer of blaOXA genes or occurrence of infection outside the hospital setting should be further investigated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Kliničke medicinske znanosti

POVEZANOST RADA