Naslov
Effect of BSA coated-silver nanoparticles on the embryonal development of Mediterranean sea urchin Paracentrotus lividus

Autori
Levak, Maja ; Burić, Petra ; Pavičić-Hamer, Dijana ; Lyons, Daniel Mark

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Archives of Industrial Hygiene and Toxicology / Durgo, Ksenija ; Pavlaković, Željana ; Herman, Makso - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2016, 32-32

Skup
5th Croatian Congress of Toxicology with International Participation

Mjesto i datum
Poreč, Hrvatska, 09.10.2016. - 12.10.2016

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
silver nanoparticles, bovine serum albumin, sea urchin, embryonal development, BSA-AgNP complex

Sažetak
In parallel with the growing use of nanoparticle-containing products, their release into the environment over the coming years is expected to increase significantly. Silver nanoparticles (AgNP) have been found to be toxic in large part due to their release of silver ions. We have found that bovine serum albumin (BSA), as a model for bio-corona-forming macromolecules, stabilizes silver colloids from agglomeration in high salinity marine waters by electrosteric repulsion for a relatively long time periods. Increasing BSA concentration was also found to reduce dissolution of silver nanoparticles in seawater with up to 3.3 times lower concentrations of silver ions noted. Paracentrotus lividus embryos were treated with BSA (0-1 mol L-1), 40 nm AgNP (50 and 100 μg L- 1) and BSA coated-AgNP complexes, 2 hours post fertilization. After 72 h the counting of normally developed pluteus (NP) larvae, retarded plutei (RP) or undeveloped embryo (UND) was carried out. The untreated embryos resulted with 90 % of normally developed plutei, whereas the addition of 50 g L-1 of AgNP resulted in 83 %, and of 100 g L-1 AgNP in only 55 % of NP. When exposed to 0.001 – 0.5 mol L-1 BSA-AgNP complexes, the percentage of NP rises to around 80 % for 50 g L-1, and even around 75 % for 100 g L-1 BSA-AgNP complexes. AgNPs were proved to be less toxic when coated with albumin, which probably served as surface passivating agent, thus reducing the ion release and consequently, the AgNP toxicity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija

POVEZANOST RADA